Comparative genomic analysis provides insights into the evolution and niche adaptation of marine Magnetospira sp QH-2 strain

被引：35

作者：

Ji, Boyang ^{[1
]}

Zhang, Sheng-Da ^{[1
]}

Arnoux, Pascal ^{[2
]}

Rouy, Zoe ^{[3
,4
,5
]}

Alberto, Francois ^{[1
]}

Philippe, Nadege ^{[1
]}

Murat, Dorothee ^{[1
]}

Zhang, Wei-Jia ^{[1
,6
,7
]}

Rioux, Jean-Baptiste

Ginet, Nicolas ^{[2
]}

Sabaty, Monique ^{[2
]}

Mangenot, Sophie ^{[8
]}

Pradel, Nathalie ^{[9
]}

Tian, Jiesheng ^{[6
,7
]}

Yang, Jing ^{[1
,6
,7
]}

Zhang, Lichen ^{[1
]}

Zhang, Wenyan

Pan, Hongmiao ^{[10
]}

Henrissat, Bernard ^{[11
]}

Coutinho, Pedro M. ^{[11
]}

Li, Ying ^{[6
,7
,12
]}

Xiao, Tian ^{[10
]}

Medigue, Claudine ^{[3
,4
,5
]}

Barbe, Valerie ^{[8
]}

Pignol, David ^{[2
,12
]}

Talla, Emmanuel ^{[1
]}

Wu, Long-Fei ^{[1
,12
]}

机构：

[1] Aix Marseille Univ, CNRS, Unite Mixte Rech 7283, Chim Bacterienne Lab, F-13402 Marseille 20, France

[2] Univ Aix Marseille, CEA DSV IBEB, CNRS CEA, UMR 7265,Lab Bioengn Cellulaire, F-13180 St Paul Les Durance, France

[3] Inst Genom Genoscope, Lab Analyse Bioinformat Genom & Metabol, F-91057 Evry, France

[4] CNRS, Unite Mixte Rech 8030, F-91057 Evry, France

[5] Univ Evry, UEVE, F-91025 Evry, France

[6] Chinese Acad Agr Sci, State Key Labs Agrobiotechnol, Beijing 100193, Peoples R China

[7] Chinese Acad Agr Sci, Coll Biol Sci, Beijing 100193, Peoples R China

[8] Inst Genom Genoscope, Lab Finit, F-91057 Evry, France

[9] Aix Marseille Univ, Univ Sud Toulon Var, CNRS INSU, IRD,MIO,UM110, F-13288 Marseille, France

[10] Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China

[11] Aix Marseille Univ, CNRS, Unite Mixte Rech 7257, F-13288 Marseille 9, France

[12] CNRS, Lab Int Assoc Biomineralisat & Nanostruct LIA Bio, F-13402 Marseille 20, France

来源：

ENVIRONMENTAL MICROBIOLOGY | 2014年 / 16卷 / 02期

关键词：

MAGNETOTACTIC BACTERIUM; IRON; SEQUENCE; BIOMINERALIZATION; PREDICTION; CYCLE; DNA; IDENTIFICATION; PERFORMANCE; PHYLOGENIES;

D O I：

10.1111/1462-2920.12180

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Magnetotactic bacteria (MTB) are capable of synthesizing intracellular organelles, the magnetosomes, that are membrane-bounded magnetite or greigite crystals arranged in chains. Although MTB are widely spread in various ecosystems, few axenic cultures are available, and only freshwater Magnetospirillum spp. have been genetically analysed. Here, we present the complete genome sequence of a marine magnetotactic spirillum, Magnetospira sp. QH-2. The high number of repeats and transposable elements account for the differences in QH-2 genome structure compared with other relatives. Gene cluster synteny and gene correlation analyses indicate that the insertion of the magnetosome island in the QH-2 genome occurred after divergence between freshwater and marine magnetospirilla. The presence of a sodium-quinone reductase, sodium transporters and other functional genes are evidence of the adaptive evolution of Magnetospira sp. QH-2 to the marine ecosystem. Genes well conserved among freshwater magnetospirilla for nitrogen fixation and assimilatory nitrate respiration are absent from the QH-2 genome. Unlike freshwater Magnetospirillum spp., marine Magnetospira sp. QH-2 neither has TonB and TonB-dependent receptors nor does it grow on trace amounts of iron. Taken together, our results show a distinct, adaptive evolution of Magnetospira sp. QH-2 to marine sediments in comparison with its closely related freshwater counterparts. © 2013 Society for Applied Microbiology and John Wiley & Sons Ltd.

引用

页码：525 / 544

页数：20