Enzymatic synthesis of monolaurin

被引：0
|
作者：
Pereira, CCB
Da Silva, MAP
Langone, MAP
机构：
[1] Univ Estado Rio De Janeiro, Inst Quim, BR-20550013 Rio De Janeiro, RJ, Brazil
[2] Univ Fed Rio de Janeiro, Escola Quim, Ctr Technol, BR-21949900 Rio De Janeiro, RJ, Brazil
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2004年 / 113卷
关键词：
monolaurin; immobilized lipase; esterification; experimental design; solvent-free medium;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of this study was to produce monolaurin utilizing a commercial immobilized lipase (Lipozyme IM-20; Novo Nordisk, Bagsvaerd, Denmark) through the direct esterification of lauric acid and glycerol in a solvent-free system. The influence of fatty acid/glycerol molar ratio, temperature, and Lipozyme (IM-20) concentration on the molar fraction of monolaurin were determined using an experimental design. The best conditions employed were 55degreesC, lauric acid/glycerol molar ratio of 1.0, and 3.0% (w/w) enzyme concentration. The final product, obtained after 6 h of reaction, was 45.5% monolaurin, 26.8% dilaurin, 3.1% trilaurin, and 24.6% lauric acid. The reusability of the enzyme was also studied.
引用
收藏
页码：433 / 445
页数：13
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