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Expression of Ethylene Receptor Gene from Oncidium 'Gower Ramsey' during Flower Senescence
被引:0
|作者:
Umikalsum, M. B.
[1
]
Abu Bakar, K.
[2
]
Faridah, Q. Z.
[3
]
机构:
[1] MARDI, Hort Res Ctr, Dept Inst, POB 12301, Kuala Lumpur 50774, Malaysia
[2] MARDI, Biotechnol Res Ctr, Dept Inst, Kuala Lumpur 50774, Malaysia
[3] Univ Putra Malaysia, Dept Biol, Serdang 43400, Malaysia
来源:
VII INTERNATIONAL POSTHARVEST SYMPOSIUM
|
2013年
/
1012卷
关键词:
real-time RT-PCR;
gene expression;
'Dancing Lady';
RT-PCR;
ARABIDOPSIS;
FAMILY;
L;
PLANTS;
ETR1;
D O I:
10.17660/ActaHortic.2013.1012.12
中图分类号:
S3 [农学(农艺学)];
学科分类号:
0901 ;
摘要:
The process of flower senescence is influenced by the plant hormone, ethylene. In Arabidopsis, ethylene perception is controlled by a family of five genes, including ETHYLENE RESPONSE 1 (ETR1), ETHYLENE RESPONSE SENSOR 1 (ERS1), ETHYLENE RESPONSE 2 (ETR2), ETHYLENE RESPONSE SENSOR 2 (ERS2) and ETHYLENE-INSENSITIVE 4 (EIN4). They fall into two subfamilies based on their sequence similarities. In flower, it has been reported that a similar set of genes is also involved. The changes in gene expression in ethylene signaling during senescence of orchids flowers were studied. Total RNA was extracted from the self-pollinated Oncidium 'Gower Ramsey' flowers followed with reverse transcriptase-polymerase chain reaction (RT-PCR). Previously, an ethylene gene named ER2(5), was cloned, which encodes for a protein that has a missing region in the histidine kinase domain as compared to the sequence of ER protein for a similar orchid hybrid. In this study, the result demonstrated that the expression of the isolated gene was successfully detected by real-time RT-PCR at a very low level in the tested flower tissues, as well as in roots. Results suggest that ER2(5) may be involved in the development of different plant tissues.
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页码:141 / 147
页数:7
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