Expression of Ethylene Receptor Gene from Oncidium 'Gower Ramsey' during Flower Senescence

被引:0
|
作者
Umikalsum, M. B. [1 ]
Abu Bakar, K. [2 ]
Faridah, Q. Z. [3 ]
机构
[1] MARDI, Hort Res Ctr, Dept Inst, POB 12301, Kuala Lumpur 50774, Malaysia
[2] MARDI, Biotechnol Res Ctr, Dept Inst, Kuala Lumpur 50774, Malaysia
[3] Univ Putra Malaysia, Dept Biol, Serdang 43400, Malaysia
来源
VII INTERNATIONAL POSTHARVEST SYMPOSIUM | 2013年 / 1012卷
关键词
real-time RT-PCR; gene expression; 'Dancing Lady'; RT-PCR; ARABIDOPSIS; FAMILY; L; PLANTS; ETR1;
D O I
10.17660/ActaHortic.2013.1012.12
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The process of flower senescence is influenced by the plant hormone, ethylene. In Arabidopsis, ethylene perception is controlled by a family of five genes, including ETHYLENE RESPONSE 1 (ETR1), ETHYLENE RESPONSE SENSOR 1 (ERS1), ETHYLENE RESPONSE 2 (ETR2), ETHYLENE RESPONSE SENSOR 2 (ERS2) and ETHYLENE-INSENSITIVE 4 (EIN4). They fall into two subfamilies based on their sequence similarities. In flower, it has been reported that a similar set of genes is also involved. The changes in gene expression in ethylene signaling during senescence of orchids flowers were studied. Total RNA was extracted from the self-pollinated Oncidium 'Gower Ramsey' flowers followed with reverse transcriptase-polymerase chain reaction (RT-PCR). Previously, an ethylene gene named ER2(5), was cloned, which encodes for a protein that has a missing region in the histidine kinase domain as compared to the sequence of ER protein for a similar orchid hybrid. In this study, the result demonstrated that the expression of the isolated gene was successfully detected by real-time RT-PCR at a very low level in the tested flower tissues, as well as in roots. Results suggest that ER2(5) may be involved in the development of different plant tissues.
引用
收藏
页码:141 / 147
页数:7
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