Molecular cloning and biochemical characterization of two UDP-glycosyltransferases from poplar

被引：21
作者：
Veljanovski, Vasko
Constabel, C. Peter
机构：
[1] Univ Victoria, Ctr Forest Biol, Victoria, BC V8W 2Y2, Canada
[2] Univ Victoria, Dept Biol, Victoria, BC V8W 2Y2, Canada
来源：
PHYTOCHEMISTRY | 2013年 / 91卷
基金：
加拿大自然科学与工程研究理事会;
关键词：
Poplar; Populus trichocarpa; Salicaceae; Flavonoid; Plant defense; Proanthocyanidin; Anthocyanin; SECONDARY METABOLISM; HYBRID POPLAR; VIGNA-MUNGO; ARABIDOPSIS; EXPRESSION; POPULUS; BIOSYNTHESIS; GLYCOSIDES; FLAVONOIDS; REVEALS;
D O I：
10.1016/j.phytochem.2012.12.012
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two pathogen-induced uridine diphosphate glycosyltransferases (UGTs) identified previously via co-expression with induced proanthocyanidin (PA) synthesis in poplar were cloned and characterized. Phylogenetic analysis grouped both genes with other known flavonoid UGTs that act on flavonols and anthocyanins. Recombinant enzymes were produced in order to test if they could glycoslate flavonoids. PtUGT78L1 accepted the flavonols quercetin and kaempferol as well as cyanidin, and used UDP-galactose as a sugar donor. PtUGT78M1 did not accept any of the flavonoids tested as a substrate, but did transfer glucose from UDP-glucose to the universal substrate 2,4,6-trichlorophenol. However, neither enzyme acted on the flavan-3-ols catechin or epicatechin, intermediates in the PA biosynthetic pathway. (C) 2013 Elsevier Ltd. All rights reserved.
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收藏
页码：148 / 157
页数：10
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