Nitric oxide and noradrenaline contribute to the temperature threshold of the axon reflex response to gradual local heating in human skin

被引:99
作者
Houghton, Belinda L. [1 ]
Meendering, Jessica R. [1 ]
Wong, Brett J. [1 ]
Minson, Christopher T. [1 ]
机构
[1] Univ Oregon, Dept Human Physiol, Eugene, OR 97403 USA
来源
JOURNAL OF PHYSIOLOGY-LONDON | 2006年 / 572卷 / 03期
关键词
D O I
10.1113/jphysiol.2005.104067
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The initial skin blood flow response to rapid local heating is an axon reflex, which may be mediated by calcitonin gene-related peptide and substance P released from C-fibres. We investigated the role of nitric oxide (NO) and noradrenaline on the temperature threshold for the axon reflex during gradual local heating. 36 subjects participated in two studies. Using microdialysis, we examined the following interventions: NO synthase inhibition (10 mM N-G-nitro-L-arginine methyl ester, L-NAME); low-dose NO infusion (1.0 mu m sodium nitroprusside, SNP); adrenergic blockade (10 mM bretylium tosylate); and low-dose (0.1 mu m) noradrenaline infusion. Laser-Doppler flowmetry was used to measure red blood cell flux. Skin was heated at a rate of 0.1 degrees C min(-1) from 33 degrees C to 40 degrees C. Compared to control skin sites, the axon reflex response was shifted to a higher temperature in 4 subjects in the L-NAME sites (control, 37.0 +/- 0.3 degrees C, n = 16; L-NAME, 39.8 +/- 0.1 degrees C, n = 4; P < 0.001) and absent in 12 subjects. The response was also absent in L-NAME plus low-dose SNP sites and not altered by low-dose SNP infusion alone. Adrenergic blockade, with and without low-dose noradrenaline infusion, also abolished the axon reflex response in all subjects. Low-dose noradrenaline infusion alone shifted the axon reflex to a significantly lower temperature threshold compared to control sites (control, 38.2 +/- 0.5 degrees C; noradrenaline, 37.7 +/- 0.4 degrees C, P < 0.05, n = 5). These results suggest that endogenous; NO and noradrenaline contribute to the temperature threshold of the axon reflex response during gradual local heating of the skin.
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收藏
页码:811 / 820
页数:10
相关论文
共 35 条
  • [1] Interactions of bradykinin and norepinephrine on rat cutaneous nociceptors in both normal and inflamed conditions in vitro
    Banik, RK
    Sato, J
    Giron, R
    Yajima, H
    Mizumura, K
    [J]. NEUROSCIENCE RESEARCH, 2004, 49 (04) : 421 - 425
  • [2] Differences between the Lewis and Sprague-Dawley rats in chronic inflammation induced norepinephrine sensitivity of cutaneous C-fiber nociceptors
    Banik, RK
    Sato, J
    Yajima, H
    Mizumura, K
    [J]. NEUROSCIENCE LETTERS, 2001, 299 (1-2) : 21 - 24
  • [3] Local hyperhemia to heating is impaired in secondary Raynaud's phenomenon
    Boignard, A
    Salvat-Melis, M
    Carpentier, PH
    Minson, CT
    Grange, L
    Duc, C
    Sarrot-Reynauld, F
    Cracowski, JL
    [J]. ARTHRITIS RESEARCH & THERAPY, 2005, 7 (05) : R1103 - R1112
  • [4] SUBSTANCE-P REGULATES THE VASODILATOR ACTIVITY OF CALCITONIN GENE-RELATED PEPTIDE
    BRAIN, SD
    WILLIAMS, TJ
    [J]. NATURE, 1988, 335 (6185) : 73 - 75
  • [5] CALCITONIN GENE-RELATED PEPTIDE IS A POTENT VASODILATOR
    BRAIN, SD
    WILLIAMS, TJ
    TIPPINS, JR
    MORRIS, HR
    MACINTYRE, I
    [J]. NATURE, 1985, 313 (5997) : 54 - 56
  • [6] Enhancement of thermal hyperalgesia by α-adrenoceptors in capsaicin-treated skin
    Drummond, PD
    [J]. JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM, 1998, 69 (2-3): : 96 - 102
  • [7] Noradrenaline provokes axon reflex hyperaemia in the skin of the human forearm
    Drummond, PD
    Lipnicki, DM
    [J]. JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM, 1999, 77 (01): : 39 - 44
  • [8] NORADRENALINE INCREASES HYPERALGESIA TO HEAT IN SKIN SENSITIZED BY CAPSAICIN
    DRUMMOND, PD
    [J]. PAIN, 1995, 60 (03) : 311 - 315
  • [9] Exogenous nitric oxide inhibits sympathetically mediated vasoconstriction in human skin
    Durand, S
    Davis, SL
    Cui, J
    Crandall, CG
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 2005, 562 (02): : 629 - 634
  • [10] CUTANEOUS VASODILATATION INDUCED BY NITRIC OXIDE-EVOKED STIMULATION OF AFFERENT NERVES IN THE RAT
    HOLZER, P
    JOCIC, M
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1994, 112 (04) : 1181 - 1187