Bergamottin, a natural furanocoumarin abundantly present in grapefruit juice, suppresses the invasiveness of human glioma cells via inactivation of Rac1 signaling

被引：14
作者：
Luo, Wenzheng [1 ]
Song, Zhenyu [1 ]
Sun, Hongwei [1 ]
Liang, Junxin [1 ]
Zhao, Shanshan [2 ]
机构：
[1] Zhengzhou Univ, Affiliated Hosp 1, Dept Neurosurg, Zhengzhou 450052, Henan, Peoples R China
[2] Zhengzhou Univ, Affiliated Hosp 1, Dept Magnet Resonance Imaging, 1 Jianshe East Rd, Zhengzhou 450052, Henan, Peoples R China
来源：
ONCOLOGY LETTERS | 2018年 / 15卷 / 03期
关键词：
glioma; invasiveness; phytochemical; Rac1; signaling; DOWN-REGULATION; INVASION; PATHWAY; EXPRESSION; CANCER; ACTIVATION; APOPTOSIS; MMP-9; INHIBITION; INITIATION;
D O I：
10.3892/ol.2017.7641
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The aim of the present study was to explore the effect of bergamottin, a natural furanocoumarin obtained from grapefruit juice, on the invasiveness of human glioma cells. The results revealed that treatment with bergamottin for 48 h significantly inhibited wound-healing migration and Matrigel invasion of human glioma cells, compared with untreated cells (P< 0.05). Bergamottin treatment caused a significant decrease in the expression and secretion of matrix metalloproteinase (MMP)-9 in glioma cells compared with untreated cells (P< 0.05). A Rac1-GTP pull-down assay demonstrated that bergamottin-treated glioma cells had a significantly decreased level of active Rac1-GTP compared with untreated cells (P< 0.05). However, bergamottin had no significant effect on cell division cycle 42 activity. Expression of constitutively activated Rac1 almost completely restored the migration and invasion of bergamottin-treated glioma cells. In addition, bergamottin-induced downregulation of MMP-9 was prevented by exogenous activated Rac1. The results of the present study demonstrated that bergamottin exhibits anti-invasive activity in human glioma cells through the inactivation of Rac1 and downregulation of MMP-9.
引用
收藏
页码：3259 / 3266
页数：8
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