Competitive binding of aqueous perfluoroctanesulfonic acid and ibuprofen with bovine serum albumin studied by electrospray ionization mass spectrometry

被引：7

作者：

D'Alessandro, Michelle L. ^{[1
]}

Ellis, David A. ^{[1
,2
]}

Carter, Jennifer A. ^{[1
]}

Stock, Naomi L. ^{[2
]}

March, Raymond E. ^{[1
,2
]}

机构：

[1] Trent Univ, Dept Chem, Peterborough, ON K9J 7B8, Canada

[2] Trent Univ, Water Qual Ctr, Peterborough, ON K9J 7B8, Canada

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 2013年 / 345卷

基金：

加拿大自然科学与工程研究理事会; 加拿大创新基金会;

关键词：

Perfluoroctanesulfonic acid; Ibuprofen; Bovine serum albumin; Electrospray ionization; Noncovalent complex; Ligand binding; PERFLUORINATED FATTY-ACIDS; PERFLUOROOCTANOIC ACID; PERFLUOROALKYL ACIDS; AFFINITY; EXPRESSION; TOXICITY; GENES; SITES; DRUGS; INDUCTION;

D O I：

10.1016/j.ijms.2012.12.012

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

Serum albumin binds reversibly with and transports endogenous and exogenous ligands, such as pharmaceuticals, whose therapeutic efficacy is activated upon liberation from the protein. Aqueous perfluoroctanesulfonic acid is a bioaccumulative, pervasive species of anthropogenic origin found in the serum of 90% of Americans that adventitiously binds with high affinity to serum albumin in the same specific site as ibuprofen; thus the conditions for competitive binding are established. This study investigated the competitive interaction between aqueous perfluoroctanesulfonic acid and ibuprofen in binding to bovine serum albumin (BSA) using electrospray ionization mass spectrometry. Perfluoroctanesulfonate was found to displace all bound ibuprofen from BSA and to prevent further ibuprofen binding when the fluorinated analyte concentration exceeds half that of ibuprofen. These observations imply that perfluo-roctanesulfonate may displace ibuprofen and similar pharmaceuticals from serum albumin in the body under physiological conditions thus altering the pharmacokinetics and deposition of such drugs. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：28 / 36

页数：9

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