Application of EPR spectroscopy to examination of free radicals in melanins from A-375 and G-361 human melanoma malignum cells

被引:16
作者
Chodurek, Ewa [1 ]
Zdybel, Magdalena [2 ]
Pilawa, Barbara [2 ]
机构
[1] Med Univ Silesia, Dept Biopharm, Sch Pharm & Lab Med, PL-41200 Sosnowiec, Poland
[2] Med Univ Silesia, Dept Biophys, Sch Pharm & Lab Med, PL-41200 Sosnowiec, Poland
关键词
free radicals; melanin; human melanoma malignum cells; 5,7-dimethoxycoumarin; valproic acid; EPR spectroscopy; DOPA-MELANIN; PARAMAGNETIC CENTERS; 5,7-DIMETHOXYCOUMARIN; MELANOGENESIS; KANAMYCIN; BINDING; DAMAGE; UV;
D O I
10.2478/v10136-012-0023-x
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Melanins are polymorphous and multifunctional biopolymers with a relatively high concentration of free radicals. EPR spectroscopy was used to study o-semiquinone free radicals in model eumelanins synthesized from 3,4-dihydroxyphenylalanine (DOPA) and tyrosine in the presence of tyrosinase, and melanins isolated from A-375 and G-361 human melanoma malignum cells exposed to two compounds: 5,7-dimethoxycoumarin (DMC) and valproic acid (VPA). Changes were determined in the concentrations of free radicals in the individual melanins from tumour cells treated with DMC and VPA. A strong decrease in the concentrations of free radicals characterizes melanins isolated from tumour cells treated together with DMC and VPA. Slow spin-lattice relaxation processes were noted in the melanins tested with homogeneous broadened EPR spectra. The EPR technique may be useful not only for the elucidation of free radicals in melanins from A-375 and G-361 cells treated with VPA and DMC but it could also be applied to establish the relationship between melanin type and the malignancy of melanoma malignum.
引用
收藏
页码:173 / 185
页数:13
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