MicroRNA-19a mediates gastric carcinoma cell proliferation through the activation of nuclear factor-κB

被引:16
作者
Yang, Fan [1 ,2 ,3 ]
Wang, Hongjian [3 ]
Jiang, Zhenyu [3 ]
Hu, Anxiang [3 ]
Chu, Lisha [3 ]
Sun, Yiling [3 ]
Han, Junqing [1 ,2 ]
机构
[1] Shandong Univ, Prov Hosp, Dept Tumor Res, Jinan 250021, Shandong, Peoples R China
[2] Shandong Univ, Therapy Ctr, Prov Hosp, Jinan 250021, Shandong, Peoples R China
[3] Tengzhou Cent Peoples Hosp, Dept Oncol, Tengzhou 277500, Shandong, Peoples R China
基金
中国国家自然科学基金;
关键词
microRNA-19a; gastric carcinoma cell; nuclear factor-kappa B activation; CANCER; EXPRESSION; PATHWAY; INHIBITOR; PROTEINS; PROMOTER; INVASION; ALLICIN; VCAM-1; ICAM-1;
D O I
10.3892/mmr.2015.4151
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
In gastric carcinoma, the nuclear factor-kappa B (NF-kappa B) signaling pathway is highly active, and the constitutive activation of NF-kappa B prompts malignant cell proliferation. MicroRNAs are considered to be important mediators in the regulation of the NF-kappa B signaling pathway. The present study predominantly focussed on the effects of microRNA (miR)-19a on NF-kappa B activation. Reverse transcription-quantitative polymerase chain reaction was used to quantify the relative levels of miR-19a in gastric carcinoma cells. MTT assays were used to determine the effect of miR-19a on cellular proliferation. To detect the activation of NF-kappa B, western blotting was performed to measure the protein levels of NF-kappa B and the products of its downstream target genes. To define the target genes, luciferase reporter assays were used. miR-19a was found to be markedly upregulated in gastric carcinoma cells. The overexpression of miR-19a resulted in proliferation and enhanced migratory capabilities of the MGC-803 gastric carcinoma cell line. The results of the western blot analysis demonstrated that the protein levels of p65 increased when the MGC-803 cells were transfected with miR-19a mimics. In addition, the downstream target genes of miR-19a, including intercellular adhesion molecule, vascular cell adhesion molecule and monocyte chemoattractant protein-1, were upregulated. The results of the luciferase assay indicated that I kappa B-a was the target gene of miR-19a. Therefore, the results of the present study suggested that miR-19a enhances malignant gastric cell proliferation by constitutively activating the NF-kappa B signaling pathway.
引用
收藏
页码:5780 / 5786
页数:7
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