Molecular cloning and characterization of a cold-induced gene encoding DRE-binding transcription factor from cold-arid adapted ecotype of Lepidium latifolium L.

The dehydration-responsive element binding (DREB) transcription factors binds to dehydration responsive element (DRE)/C-repeat (CRT) cis-element, induces a set of abiotic stress-related genes and imparts stress endurance to plants. In the present study, a DREB1 like gene, designated as LlDREB1A, was isolated from highly cold adapted ecotype of Lepidium latifolium L. Its full-length cDNA (JN561592) sequence (925 bp) consisted of a 642 bp ORF, encoding a protein of 213 aa with deduced mol mass of 23.98 kDa and a theoretical pI of 4.81. LlDREB1A protein consisted of a typical AP2 domain, DREB1 type nuclear localization signal (NLS), C-terminal acidic domain and other signature sequences. Phylogenetic analysis suggested that it is genetically divergent from both monocot as well as dicot plants. Semi quantitative RT-PCR analysis revealed that this gene is up-regulated in response to cord stress but remained unchanged in response to either salt or drought stress. Further, functional validation of LlDRE1A gene may help its deployment in crop plants using genetic engineering approach for development of cultivars tolerant to abiotic stresses.