Isolation and characterization of 40 polymorphic microsatellite markers from Parambassis siamensis

被引：2

作者：

Koizumi, Noriyuki ^{[1
]}

Morioka, Shinsuke ^{[2
]}

Quinn, Thomas W. ^{[3
]}

Mori, Atsushi ^{[1
]}

Vongvichith, Bounsong ^{[4
]}

Nishida, Kazuya ^{[1
]}

Watabe, Keiji ^{[1
]}

Takemura, Takeshi ^{[1
]}

机构：

[1] Natl Agr & Food Res Org, Inst Rural Engn, Tsukuba, Ibaraki 3058609, Japan

[2] Japan Int Res Ctr Agr Sci, Tsukuba, Ibaraki 3058686, Japan

[3] Univ Denver, Dept Biol Sci, Denver, CO 80208 USA

[4] Living Aquat Resources Res Ctr, Viangchan 9108, Laos

来源：

CONSERVATION GENETICS RESOURCES | 2012年 / 4卷 / 04期

基金：

日本学术振兴会;

关键词：

Ambassidae; Microsatellite; Genetic diversity; Agricultural canal; Rice paddy ecosystem; SOFTWARE;

D O I：

10.1007/s12686-012-9700-z

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

We isolated and characterized a total of 40 polymorphic microsatellite loci from the genome of Parambassis siamensis. Flanking Polymerase Chain Reaction primers were developed and used to show that all loci were polymorphic with the number of observed alleles per locus in 32 individuals ranging from 2 to 29. The observed heterozygosity ranged from 0.063 to 0.969, while the expected heterozygosity varied from 0.062 to 0.960. All loci conformed to Hardy-Weinberg equilibrium, none of the loci combinations showed significant linkage disequilibrium, and no loci showed evidence of null alleles. These 40 microsatellite loci should be useful for conducting future investigations into the genetic differentiation and structure of populations of P. siamensis.

引用

页码：1031 / 1035

页数：5

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