Complete genome sequence of acute viral necrosis virus associated with massive mortality outbreaks in the Chinese scallop, Chlamys farreri

被引：78

作者：

Ren, Weicheng ^{[1
,2
]}

Chen, Haixia ^{[3
]}

Renault, Tristan ^{[4
]}

Cai, Yuyong ^{[1
]}

Bai, Changming ^{[1
]}

Wang, Chongming ^{[1
]}

Huang, Jie ^{[1
]}

机构：

[1] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Maricultural Organism Dis Control & Pathogen Mol, Qingdao 266071, Peoples R China

[2] Univ Gothenburg, Dept Rheumatol & Inflammat, S-40530 Gothenburg, Sweden

[3] Univ Gothenburg, Dept Biol & Environm Sci, S-40530 Gothenburg, Sweden

[4] Ifremer, Unite Sante Genet & Microgiol Mollusques, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France

来源：

VIROLOGY JOURNAL | 2013年 / 10卷

基金：

国家高技术研究发展计划(863计划);

关键词：

Acute viral necrosis virus (AVNV); Herpesvirus; OsHV-1; Genome; HERPES-LIKE-VIRUS; HATCHERY-REARED LARVAE; CRASSOSTREA-GIGAS; PACIFIC OYSTERS; OSTREID HERPESVIRUS-1; INTERSPECIES TRANSMISSION; REPLICATION; ORIGIN; GANGLIONEURITIS; PURIFICATION;

D O I：

10.1186/1743-422X-10-110

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Acute viral necrosis virus (AVNV) is the causative agent of a serious disease resulting in high mortality in cultured Chinese scallops, Chlamys farreri. We have sequenced and analyzed the complete genome of AVNV. Results: The AVNV genome is a linear, double-stranded DNA molecule of 210,993 bp with a nucleotide composition of 38.5% G + C. A total of 123 open reading frames were predicted to encode functional proteins, ranging from 41 to 1,878 amino acid residues. The DNA sequence of AVNV is 97% identical to that of ostreid herpesvirus 1 (OsHV-1), and the amino acid sequences of the encoded proteins of these two viruses are 94-100% identical. The genomic organization of AVNV is similar to that of OsHV-1, and consists of two unique regions (170.4 kb and 3.4 kb, respectively), each flanked by two inverted repeats (7.6 kb and 10.2 kb, respectively), with a third unique region (1.5 kb) situated between the two internal repeats. Conclusions: Our results indicate that AVNV is a variant of OsHV-1. The AVNV genome sequence provides information useful for understanding the evolution and divergence of OsHV-1 in marine molluscs.

引用

页数：7