Kinetics and Thermodynamics of 1-anilino-8-naphthalene Sulfonate Interactions with Urinary Trypsin Inhibitor

被引：3

作者：

Zuo, Zhenyu ^{[1
]}

Fan, Handong

Guo, Jianjun

Zhou, Wei ^{[1
]}

Li, Lingling ^{[1
]}

机构：

[1] Wuhan Univ Sci & Technol, Coll Chem Engn & Technol, Wuhan 430081, Peoples R China

来源：

PROTEIN JOURNAL | 2012年 / 31卷 / 07期

关键词：

Urinary trypsin inhibitor; 1-Anilino-8-naphthalene sulfonate; Fluorescence spectrum; Isothermal titration calorimetry; Molecular modeling; OVARIAN-CANCER CELLS; BINDING; PROTEIN; STABILITY; ACID;

D O I：

10.1007/s10930-012-9443-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interactions between Urinary Trypsin Inhibitor (UTI) and 1-anilino-8-naphthalene sulfonate (ANS) were investigated by fluorescence spectra, isothermal titration calorimetry and molecular modeling. The results revealed the presence of four specific binding sites for ANS on UTI, with interactions driven mainly by electrostatic forces. The four specific binding sites indicated the involvement of four hydrophobic patches on UTI. Experimental data also confirmed the presence of a further five nonspecific binding sites that interacted mainly by the formation of salt bridges between the sulfonates of ANS and positive residues on the surface of UTI.

引用

页码：585 / 591

页数：7

共 13 条

[1] Kinetics and Thermodynamics of 1-anilino-8-naphthalene Sulfonate Interactions with Urinary Trypsin Inhibitor
Zhenyu Zuo
Handong Fan
Jianjun Guo
Wei Zhou
Lingling Li
The Protein Journal, 2012, 31 : 585 - 591
[2] Kinetics and thermodynamics of the interaction of 1-anilino-naphthalene-8-sulfonate with proteins
Cattoni, Diego I.
Kaufman, Sergio B.
Gonzalez Flecha, F. Luis
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2009, 1794 (11): : 1700 - 1708
[3] 1-Anilino-8-Naphthalene Sulfonate (ANS) Is Not a Desirable Probe for Determining the Molten Globule State of Chymopapain
Qadeer, Atiyatul
Rabbani, Gulam
Zaidi, Nida
Ahmad, Ejaz
Khan, Javed M.
Khan, Rizwan H.
PLOS ONE, 2012, 7 (11):
[4] Effect of carbonylcyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) on the interaction of 1-anilino-8-naphthalene sulfonate (ANS) with phosphatidylcholine liposomes
Cutro, Andrea C.
Montich, Guillermo G.
Roveri, Oscar A.
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 2014, 46 (02) : 119 - 125
[5] Kinetics of urinary trypsin inhibitor in patients undergoing partial hepatectomy
Noie, T
Sugawara, Y
Harihara, Y
Takayama, T
Kubota, K
Ohashi, Y
Makuuchi, M
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 2001, 36 (04) : 410 - 416
[6] Elucidating the binding thermodynamics of 8-anilino-1-naphthalene sulfonic acid with the A-state of α-lactalbumin:: An isothermal titration calorimetric investigation
Singh, Sreelekha K.
Kishore, Nand
BIOPOLYMERS, 2006, 83 (03) : 205 - 212
[7] New insight on 8-anilino-1-naphthalene sulfonic acid interaction with TgFNR for hydrophobic exposure analysis
Singh, Kulwant
Hussain, Islam
Mishra, Vibhor
Akhtar, Md. Sohail
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 122 : 636 - 643
[8] 1-Anilino-8-naphtalene sulfonate probes a gastric HK-ATPase potassium site whose access requires ionophores
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Lins, L
Gallet, X
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Thomas-Soumarmon, A
JOURNAL OF MEMBRANE BIOLOGY, 1998, 165 (02): : 153 - 160
[9] Impedimetric sensor for tyramine based on gold nanoparticle doped-poly(8-anilino-1-naphthalene sulphonic acid) modified gold electrodes
da Silva, Wanderson
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Ajayi, Rachel F.
Iwuoha, Emmanuel I.
Brett, Christopher M. A.
TALANTA, 2019, 195 : 604 - 612
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Kato, S
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Hino, T
Takahashi, H
Tomoike, H
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1997, 112 (02) : 157 - 162

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