A SPRY domain-containing SOCS box protein 3 (SPSB3) involved in the regulation of cytokine production in granulocytes of Crassostrea gigas

被引：6

作者：

Wang, Min ^{[1
,3
]}

Liu, Chang ^{[1
,3
,4
]}

Wang, Weilin ^{[1
,2
,3
,4
]}

Dong, Miren ^{[1
]}

Zhang, Peng ^{[1
]}

Liu, Yu ^{[1
]}

Wang, Lingling ^{[1
,2
,3
,4
]}

Song, Linsheng ^{[1
,2
,3
]}

机构：

[1] Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China

[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China

[3] Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China

[4] Dalian Ocean Univ, Dalian Key Lab Dis Control & Prevent Aquaculture, Dalian 116023, Peoples R China

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2019年 / 95卷

关键词：

Crassostrea gigas; SPRY domain-containing SOCS box protein 3 (SPSB3); Immune response; Granulocytes; Cytokine production; C-TYPE LECTIN; STRUCTURAL BASIS; VIBRIO-SPLENDIDUS; GENE-EXPRESSION; RECOGNITION; OYSTER; MOTIF; ACTIVATION; IRF-3; BINDS;

D O I：

10.1016/j.dci.2019.01.013

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The sp1A/ryanodine receptor (SPRY) family members have been reported to involve in important biological pathways, including innate immune signaling, cytokine signaling suppression, development, cell growth, and retroviral restriction. In the present study, a SPRY domain-containing SOCS box protein (named as CgSPSB3) was identified and characterized from oyster Crassostrea gigas. The open reading frame of CgSPSB3 gene was of 699 bp, encoding a polypeptide of 232 amino acid residues with a central SPRY domain and a C-terminal SOCS box motif. CgSPSB3 mRNA transcripts could be detected in all the examined tissues with the highest level in hemocytes, which was about 82.72-fold (p < 0.05) of that in gonad. Furthermore, the expression level of CgSPSB3 mRNA in granulocytes was significantly higher than that in semi-granulocytes and agranulocytes, which was about 2.04-fold (p < 0.05) of the average level of hemocytes. Immunofluorescence assay further revealed that CgSPSB3 protein was mainly distributed in the cytoplasm of granulocytes. The mRNA expression of CgSPSB3 in hemocytes was up-regulated after lipopolysaccharide (LPS) and Vibrio splendidus stimulations. The mRNA expression of CgIFNLP, CgIL17-5 and CgTNF-1 decreased significantly (p < 0.05) at 24 h after the CgSPSB3 mRNA was knocked down by RNAi. These results collectively indicated that CgSPSB3 might play an important role in regulating cytokines production in granulocytes of C. gigas.

引用

页码：28 / 37

页数：10

共 35 条

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