The Role of Endogenous Reactive Oxygen Species in Cardiac Myocyte Autophagy

被引:13
作者
Wang, Jia-Pu [1 ,2 ,3 ]
Chi, Rui-Fang [1 ,2 ,3 ]
Liu, Jia [2 ,3 ]
Deng, Yong-Zhi [2 ,3 ]
Han, Xue-Bin [2 ,3 ]
Qin, Fu-Zhong [1 ,2 ,3 ]
Li, Bao [1 ,4 ]
机构
[1] Shanxi Med Univ, 56 South Xinjian Rd, Taiyuan 030001, Shanxi, Peoples R China
[2] Shanxi Med Univ, Affiliated Cardiovasc Hosp, 18 Yifen St, Taiyuan 030024, Shanxi, Peoples R China
[3] Shanxi Prov Cardiovasc Hosp, Taiyuan, Shanxi, Peoples R China
[4] Shanxi Med Univ, Hosp 2, Taiyuan, Shanxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Reactive oxygen species; Endogenous; Autophagy; Cardiomyocytes; Rats; CONTRACTILE DYSFUNCTION ROLE; ACTIVATED PROTEIN-KINASE; OXIDATIVE STRESS; CARDIOMYOCYTE; REPERFUSION; PHYSIOLOGY; APOPTOSIS; ISCHEMIA; HEART;
D O I
10.33549/physiolres.933653
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Autophagy is implicated in the maintenance of cardiac homeostasis. Autophagy is activated in heart failure, in which reactive oxygen species (ROS) are increased. Exogenous ROS have been shown to induce cardiomyocyte autophagy alterations. However, little is known about the influences of physiological levels of endogenous ROS on cardiomyocyte autophagy. In the present study, we tested the hypothesis that endogenous ROS in cardiomyocytes play an important role in inducing autophagy. Cultured H9C2 cardiomyocytes or Sprague-Dawley rats were treated with the antioxidant N-acetyl-cysteine (NAC) or the superoxide dismutase mimic tempol under the basal or nutrient deprivation conditions. The autophagic flux was assessed by the lysosomal inhibitor chloroquine. In H9C2 cardiomyocytes, under a basal condition, NAC or tempol increased the ratio of LC3 II/I proteins and reduced LC3 II autophagic flux. Under nutrient deprivation, NAC increased the LC3 II/I ratio and reduced LC3 II autophagic flux. In vivo studies in rats, NAC treatment increased the LC3 II/I ratio and p-Akt protein expression in myocardium. We concluded that the antioxidants reduced autophagic flux in cardiomyocytes under the basal or nutrient deprivation conditions, suggesting that endogenous ROS promote autophagy flux under physiological conditions, and this effect is mediated, at least in part, through Akt inhibition.
引用
收藏
页码:31 / 40
页数:10
相关论文
共 23 条
[1]   Redox Signaling in Cardiac Physiology and Pathology [J].
Burgoyne, Joseph R. ;
Mongue-Din, Heloise ;
Eaton, Philip ;
Shah, Ajay M. .
CIRCULATION RESEARCH, 2012, 111 (08) :1091-1106
[2]   Adiponectin Modulates Oxidative Stress-Induced Autophagy in Cardiomyocytes [J].
Essick, Eric E. ;
Wilson, Richard M. ;
Pimentel, David R. ;
Shimano, Masayuki ;
Baid, Simoni ;
Ouchi, Noriyuki ;
Sam, Flora .
PLOS ONE, 2013, 8 (07)
[3]   Involvement of the nicotinamide adenosine dinucleotide phosphate oxidase isoform Nox2 in cardiac contractile dysfunction occurring in response to pressure overload [J].
Grieve, DJ ;
Byrne, JA ;
Siva, A ;
Layland, J ;
Johar, S ;
Cave, AC ;
Shah, AM .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2006, 47 (04) :817-826
[4]   Oxidative Stress Stimulates Autophagic Flux During Ischemia/Reperfusion [J].
Hariharan, Nirmala ;
Zhai, Peiyong ;
Sadoshima, Junichi .
ANTIOXIDANTS & REDOX SIGNALING, 2011, 14 (11) :2179-2190
[5]   Oxidative stress induces GLUT4 translocation by activation of P13-K/Akt and dual AMPK kinase in cardiac myocytes [J].
Horie, Takahiro ;
Ono, Koh ;
Nagao, Kazuya ;
Nishi, Hitoo ;
Kinoshita, Minako ;
Kawamura, Teruhisa ;
Wada, Hiromichi ;
Shimatsu, Akira ;
Kita, Toru ;
Hasegawa, Koji .
JOURNAL OF CELLULAR PHYSIOLOGY, 2008, 215 (03) :733-742
[6]   Eicosapentaenoic acid attenuated oxidative stress-induced cardiomyoblast apoptosis by activating adaptive autophagy [J].
Hsu, Hsiu-Ching ;
Chen, Ching-Yi ;
Chiang, Chun-Hsien ;
Chen, Ming-Fong .
EUROPEAN JOURNAL OF NUTRITION, 2014, 53 (02) :541-547
[7]   Chronic akt activation accentuates aging-induced cardiac hypertrophy and myocardial contractile dysfunction: role of autophagy [J].
Hua, Yinan ;
Zhang, Yingmei ;
Ceylan-Isik, Asli F. ;
Wold, Loren E. ;
Nunn, Jennifer M. ;
Ren, Jun .
BASIC RESEARCH IN CARDIOLOGY, 2011, 106 (06) :1173-1191
[8]   The role of autophagy emerging in postinfarction cardiac remodelling [J].
Kanamori, Hiromitsu ;
Takemura, Genzou ;
Goto, Kazuko ;
Maruyama, Rumi ;
Tsujimoto, Akiko ;
Ogino, Atsushi ;
Takeyama, Toshiaki ;
Kawaguchi, Tomonori ;
Watanabe, Takatomo ;
Fujiwara, Takako ;
Fujiwara, Hisayoshi ;
Seishima, Mitsuru ;
Minatoguchi, Shinya .
CARDIOVASCULAR RESEARCH, 2011, 91 (02) :330-339
[9]   Mitochondria and Mitophagy The Yin and Yang of Cell Death Control [J].
Kubli, Dieter A. ;
Gustafsson, Asa B. .
CIRCULATION RESEARCH, 2012, 111 (09) :1208-1221
[10]   Cardiovascular autophagy Concepts, controversies, and perspectives [J].
Lavandero, Sergio ;
Troncoso, Rodrigo ;
Rothermel, Beverly A. ;
Martinet, Wim ;
Sadoshima, Junichi ;
Hill, Joseph A. .
AUTOPHAGY, 2013, 9 (10) :1455-1466