Duplex PCR to detect both Papaya ring spot virus and Papaya leaf curl virus simultaneously from naturally infected papaya (Carica papaya L.)

被引：0

作者：

Usharani, T. R. ^{[1
]}

Laxmi, V. ^{[2
]}

Jalali, S. ^{[2
]}

Krishnareddy, M. ^{[2
]}

机构：

[1] Indian Inst Hort Res, Div Biotechnol, Bangalore 560065, Karnataka, India

[2] Indian Inst Hort Res, Div Plant Pathol, Bangalore 560065, Karnataka, India

来源：

INDIAN JOURNAL OF BIOTECHNOLOGY | 2013年 / 12卷 / 02期

关键词：

Duplex PCR; Papaya ring spot virus (PRSV); Papaya leaf curl virus (PaLCV); COAT PROTEIN; POTYVIRUS; STRAINS; DISEASE;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Papaya, a major fruit crop in India and worldwide, is affected by many fungal and viral diseases. A mixed infection of Papaya ring spot virus (PRSV), a linear single-stranded (+) RNA genome of approx 10 kb size, and Papaya leaf curl virus (PaLCV), a bipartite Gemini virus (component A & B) having circular single-stranded DNA (+) genome of about 5.2 kb, has hampered the production and productivity of papaya in many parts of world. Rapid detection techniques are important in prevention of spread of the disease in field conditions. In the present study, a rapid and reliable PCR based detection protocol has been standardized. Sets, of primers were designed, based on the respective virus isolate sequence data available in GenBank, to obtain anticipated products of calculated size.

引用

页码：269 / 272

页数：4