Effect of Anluohuaxian Tablet Combined with γ-IFN on Schistosomal Liver Fibrosis

被引：9

作者：

Huang, Jiaquan ^{[1
]}

Huang, Haiyan ^{[1
,2
]}

Jiao, Yuntao ^{[1
]}

Ai, Guo ^{[1
]}

Huang, Tiejun ^{[1
]}

Li, Lan ^{[1
]}

Yu, Haijing ^{[1
]}

Ma, Ke ^{[1
]}

Xiao, Fei ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Infect Dis, Wuhan 430030, Peoples R China

[2] Xianning First Peoples Hosp, Dept Infect Dis, Xianning 437000, Peoples R China

来源：

JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES | 2009年 / 29卷 / 01期

关键词：

Schistosomiasis japonica; liver fibrosis; anluohuaxian tablet; gamma-interferon; hepatic pigmentation; TIMP-1; TGF-beta; 1; mRNA; TISSUE INHIBITOR; METALLOPROTEINASE-1;

D O I：

10.1007/s11596-009-0111-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The therapeutic effects of anluohuaxian tablet combined with gamma-IFN on schistosomal liver fibrosis and its mechanism were studied in a murine model and clinical cases of schistosomal liver fibrosis. Fifty Kunming mice were randomly divided into 5 groups: normal control group, infection control group, anluohuaxian tablet-treated group, gamma-IFN-treated group and combined treatment (anluohuaian tablet+gamma-IFN) group. Pathologic changes in liver, including hepatic pigmentation and the size of schistosomal egg granuloma, were observed by HE staining after treatment for 8 weeks. The expression of the type. and collagen., and TIMP-1 was detected by immunohistochemistry. TGF-beta 1 mRNA expression was examined by real-time fluorescent quantitative PCR. Sixty patients with schistosomal liver fibrosis were divided into treatment group and control group. The patients in treatment group were treated with anluohuaxian tablet in combination with gamma-IFN for 6 months. Before and after treatment, the changes of symptoms and signs, liver function, serum liver fibrosis indexes and imaging indexes were observed. The results showed that as compared with infection control group, all forms of treatments relieved the hepatic pathological injury with apparently diminished size of schistosomal egg nodules and decreased percentage of pigmentation (P<0.05). Furthermore, the expression of collagen. and, TIMP-1, and TGF-beta 1 mRNA in combined treatment group was significantly decreased as compared with anluohuaxian tablet-treated and gamma-IFN treated groups (P<0.05). In the clinical observation, the serum liver fibrosis indexes, the portal vein width as well as the spleen thickness was significantly reduced in treatment group as compared with control group (P<0.05). It was concluded that the combined use of anluohuaxian tablet with gamma-IFN in schistosomal liver fibrosis could protect liver function, alleviate liver fibrosis, and could be used as a choice in treating patients with schiatosomal liver fibrosis.

引用

页码：53 / 58

页数：6

共 15 条

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