Effect of surface hydrophobicity/hydrophilicity of mesoporous supports on the activity of immobilized lipase

被引：61

作者：

He, Jing ^{[1
]}

Xu, Yan ^{[1
]}

Ma, Hui ^{[1
]}

Zhang, Qiong ^{[1
]}

Evans, David G. ^{[1
]}

Duan, Xue ^{[1
]}

机构：

[1] Beijing Univ Chem Technol, Key Lab Sci & Technol Controllable Chem React, Beijing 100029, Peoples R China

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2006年 / 298卷 / 02期

基金：

中国国家自然科学基金;

关键词：

hydrophobicity/hydrophilicity; mesoporous support; lipase immobilization; grafting;

D O I：

10.1016/j.jcis.2005.12.063

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Taking advantage of the virtue of hydrophilic surface, lipase was firstly immobilized on SBA-15 as a support. Then the surface of the SBA-15 with enzyme entrapped inside the channels was modified by grafting with organic moieties. It has been found that the silylation with n-decyltrimethoxysilane (DE) and 3-(trimethoxysilyl)propyl methacrylate (MA) following the lipase immobilization increases the surface hydrophobicity. But the surface modified by MA shows more hydrophilicity than that modified by DE. The activity assay indicates that the hydrolytic activity for the hydrolysis of insoluble or partly soluble substrates increases with enhanced surface hydrophobicity. (c) 2006 Published by Elsevier Inc.

引用

页码：780 / 786

页数：7

共 34 条

[1] Lipase immobilisation: an equilibrium study of lipases immobilised on hydrophobic and hydrophilic/hydrophobic supports [J].

Al-Duri, B ;

Yong, YP .

BIOCHEMICAL ENGINEERING JOURNAL, 2000, 4 (03) :207-215

[2] Immobilization and characterization of porcine pancreas lipase [J].

Bagi, K ;

Simon, LM ;

Szajani, B .

ENZYME AND MICROBIAL TECHNOLOGY, 1997, 20 (07) :531-535

[3] Bioreactors with immobilized lipases: State of the art [J].

Balcao, VM ;

Paiva, AL ;

Malcata, FX .

ENZYME AND MICROBIAL TECHNOLOGY, 1996, 18 (06) :392-416

[4]

Beisson F, 2000, EUR J LIPID SCI TECH, V102, P133, DOI 10.1002/(SICI)1438-9312(200002)102:2<133::AID-EJLT133>3.3.CO

[5]

2-O

[6] STRUCTURAL CHARACTERIZATION OF (3-AMINOPROPYL)TRIETHOXYSILANE-MODIFIED SILICAS BY SI-29 AND C-13 NUCLEAR MAGNETIC-RESONANCE [J].

CARAVAJAL, GS ;

LEYDEN, DE ;

QUINTING, GR ;

MACIEL, GE .

ANALYTICAL CHEMISTRY, 1988, 60 (17) :1776-1786

[7] IMMOBILIZATION OF CANE AMYLASE AND ACID-PHOSPHATASE ON TRICALCIUM PHOSPHATE (TCP) GEL [J].

DAS, G ;

PRABHU, KA .

ENZYME AND MICROBIAL TECHNOLOGY, 1990, 12 (08) :625-630

[8] Enzyme immobilization in MCM-41 molecular sieve [J].

Diaz, JF ;

Balkus, KJ .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 1996, 2 (2-3) :115-126

[9] New supports for enzyme immobilization based on the copolymers of poly(vinylene carbonate) and α-(2-aminoethylene amino)-ω-(2-aminoethylene amino)-poly(ethylene oxide) [J].

Ding, LH ;

Qu, BJ .

REACTIVE & FUNCTIONAL POLYMERS, 2001, 49 (01) :67-76

[10] Immobilization of lipases by selective adsorption on hydrophobic supports [J].

Fernandez-Lafuente, R ;

Armisén, P ;

Sabuquillo, P ;

Fernández-Lorente, G ;

Guisán, JM .

CHEMISTRY AND PHYSICS OF LIPIDS, 1998, 93 (1-2) :185-197

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