Haemophilus influenzae and Vibrio cholerae genes for mutH are able to fully complement a mutH defect in Escherichia coli

被引：8

作者：

Friedhoff, P ^{[1
]}

Sheybani, B ^{[1
]}

Thomas, E ^{[1
]}

Merz, C ^{[1
]}

Pingoud, A ^{[1
]}

机构：

[1] Univ Giessen, Inst Biochem, D-35392 Giessen, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 208卷 / 01期

关键词：

mismatch repair; endonuclease; genetic complementation; Vibrio cholerae; Haemophilus influenzae;

D O I：

10.1016/S0378-1097(01)00588-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

MutH, MutL and MutS are essential components of the mismatch repair system in Escherichia coli. Whereas mutS and mutL genes are found in most organisms, the mutH gene is limited to some proteobacteria. We show here that the cloned genes of MutH from Vibrio cholerae and Haemophilus influenzae are able to fully complement a mutH defect in E. coli. Moreover, the purified proteins were shown to be dam methylation sensitive endonucleases, which can be activated by the E coli MutL protein. These results allow to narrow down regions that are important for the interaction of MutH with MutL. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：123 / 128

页数：6