High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus

被引：187

作者：

Zheng, Wenming ^{[1
,2
,3
,4
]}

Huang, Lili ^{[1
,2
]}

Huang, Jinqun ^{[5
]}

Wang, Xiaojie ^{[1
,2
]}

Chen, Xianming ^{[6
,7
]}

Zhao, Jie ^{[1
,2
]}

Guo, Jun ^{[1
,2
]}

Zhuang, Hua ^{[1
,2
]}

Qiu, Chuangzhao ^{[5
]}

Liu, Jie ^{[1
,2
]}

Liu, Huiquan ^{[1
,2
]}

Huang, Xueling ^{[1
,2
]}

Pei, Guoliang ^{[1
,2
]}

Zhan, Gangming ^{[1
,2
]}

Tang, Chunlei ^{[1
,2
]}

Cheng, Yulin ^{[1
,2
]}

Liu, Minjie ^{[1
,2
]}

Zhang, Jinshan ^{[1
,2
]}

Zhao, Zhongtao ^{[1
,2
]}

Zhang, Shijie ^{[1
,2
]}

Han, Qingmei ^{[1
,2
]}

Han, Dejun ^{[1
,2
]}

Zhang, Hongchang ^{[1
,2
]}

Zhao, Jing ^{[1
,2
]}

Gao, Xiaoning ^{[1
,2
]}

Wang, Jianfeng ^{[1
,2
]}

Ni, Peixiang ^{[5
]}

Dong, Wei ^{[5
]}

Yang, Linfeng ^{[5
]}

Yang, Huanming ^{[5
]}

Xu, Jin-Rong ^{[8
]}

Zhang, Gengyun ^{[5
]}

Kang, Zhensheng ^{[1
,2
]}

机构：

[1] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China

[2] Northwest A&F Univ, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China

[3] Henan Agr Univ, Key Lab Physiol Ecol & Genet Improvement Food Cro, Zhengzhou 450002, Henan Province, Peoples R China

[4] Henan Agr Univ, Coll Life Sci, Zhengzhou 450002, Henan Province, Peoples R China

[5] BGI Shenzhen, State Key Lab Agr Genom, Shenzhen 518083, Peoples R China

[6] Washington State Univ, USDA ARS, Pullman, WA 99164 USA

[7] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA

[8] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA

来源：

NATURE COMMUNICATIONS | 2013年 / 4卷

关键词：

AMINO-ACID TRANSPORTER; F-SP TRITICI; PUCCINIA-STRIIFORMIS; PLANT; SEQUENCE; HAUSTORIA; OBLIGATE; LIFE; IDENTIFICATION; ADAPTATION;

D O I：

10.1038/ncomms3673

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat. Here we report a 110-Mb draft sequence of Pst isolate CY32, obtained using a 'fosmid-to-fosmid' strategy, to better understand its race evolution and pathogenesis. The Pst genome is highly heterozygous and contains 25,288 protein-coding genes. Compared with non-obligate fungal pathogens, Pst has a more diverse gene composition and more genes encoding secreted proteins. Re-sequencing analysis indicates significant genetic variation among six isolates collected from different continents. Approximately 35% of SNPs are in the coding sequence regions, and half of them are non-synonymous. High genetic diversity in Pst suggests that sexual reproduction has an important role in the origin of different regional races. Our results show the effectiveness of the 'fosmid-to-fosmid' strategy for sequencing dikaryotic genomes and the feasibility of genome analysis to understand race evolution in Pst and other obligate pathogens.

引用

页数：10

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