Mediated xanthine oxidase potentiometric biosensors for hypoxanthine based on ferrocene carboxylic acid modified electrode

被引：37

作者：

Lawal, Abdulazeez T. ^{[1
]}

Adeloju, Samuel B. ^{[1
]}

机构：

[1] Monash Univ, Sch Appl Sci & Engn, NanoSci & Sensor Technol Res Group, Churchill, Vic 3842, Australia

来源：

FOOD CHEMISTRY | 2012年 / 135卷 / 04期

关键词：

Xanthine; Oxidase; Hypoxanthine; Fish; Biosensor; Potentiometry; Immobilisation; LIQUID-CHROMATOGRAPHIC DETERMINATION; FLOW-INJECTION ANALYSIS; AMPEROMETRIC BIOSENSOR; URIC-ACID; FISH; FRESHNESS; SENSOR; NUCLEOTIDES; INOSINE; GLUCOSE;

D O I：

10.1016/j.foodchem.2012.07.052

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A potentiometric enzyme electrode for detection of hypoxanthine (Hx) in fish meat is described. The sensor was developed by entrapment of xanthine oxidase (XOD) and ferrocene carboxylic acid (Fc) into polypyrrole (PPy) film during galvanostatic polymerisation film formation. The responses for Hx were obtained in 0-05 M phosphate buffer (pH 7.1) at 0.0 mV vs Ag/AgCl (3 M KCl). The optimum condition for the formation of PPy-XOD-Fc film include 0.4 M PPy, 6.2 U/mL XOD, 40 mM Fc, polymerisation time of 200 s and applied current density of 0-5 mA cm(-2). The sensor provides a linear response to Hx in concentration range of 5-20 mu M, (r = 0.998) and was successfully used for determination of Hx in fish. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：2982 / 2987

页数：6

共 45 条

[1] Fabrication of ultra-thin polypyrrole-glucose oxidase film from supporting electrolyte-free monomer solution for potentiometric biosensing of glucose [J].

Adeloju, SB ;

Moline, AN .

BIOSENSORS & BIOELECTRONICS, 2001, 16 (03) :133-139

[2] Amperometric biosensor for hypoxanthine based on immobilized xanthine oxidase on nanocrystal gold-carbon paste electrodes [J].

Agüi, L ;

Manso, J ;

Yáñez-Sedeño, P ;

Pingarrón, JM .

SENSORS AND ACTUATORS B-CHEMICAL, 2006, 113 (01) :272-280

[3] DETERMINATION OF HYPOXANTHINE AND XANTHINE IN PLASMA SEPARATED BY THIN-LAYER CHROMATOGRAPHY [J].

AKAOKA, I ;

NISHIZAWA, T ;

NISHIDA, Y .

BIOCHEMICAL MEDICINE, 1975, 14 (03) :285-289

[4] INVESTIGATION OF THE BATCH INJECTION-ANALYSIS TECHNIQUE WITH AMPEROMETRIC BIOCATALYTIC ELECTRODES USING A MODIFIED SMALL-VOLUME CELL [J].

AMINE, A ;

KAUFFMANN, JM ;

PALLESCHI, G .

ANALYTICA CHIMICA ACTA, 1993, 273 (1-2) :213-218

[5] An amperometric biosensor for xanthine determination prepared from xanthine oxidase immobilized in polypyrrole film [J].

Arslan, F ;

Yasar, A ;

Kiliç, E .

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, 2006, 34 (01) :113-128

[6] Immobilization of xanthine oxidase and its use in the quantitation of hypoxanthine in fish muscle tissue extracts using a flow injection method [J].

Balladin, DA ;

Narinesingh, D ;

Stoute, VA ;

Ngo, TT .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1997, 62 (2-3) :317-328

[7] Synergistic efficiency of combined HALS-UV absorber polymerizable stabilizers [J].

Bojinov, Vladimir .

JOURNAL OF APPLIED POLYMER SCIENCE, 2006, 102 (03) :2408-2415

[8]

BURNS BG, 1985, J ASSOC OFF ANA CHEM, V68, P444

[9] Amperometric detection of uric acid and hypoxanthine with Xanthine oxidase immobilized and carbon based screen-printed electrode. Application for fish freshness determination [J].

Carsol, MA ;

Volpe, G ;

Mascini, M .

TALANTA, 1997, 44 (11) :2151-2159

[10] THIN-FILM POLYAMINE BIOSENSOR - SUBSTRATE-SPECIFICITY AND APPLICATION TO FISH FRESHNESS DETERMINATION [J].

CHEMNITIUS, GC ;

SUZUKI, M ;

ISOBE, K ;

KIMURA, J ;

KARUBE, I ;

SCHMID, RD .

ANALYTICA CHIMICA ACTA, 1992, 263 (1-2) :93-100

← 1 2 3 4 5 →