Study on in-vitro Biochemical Growth Characterization and Assessment of Hemolytic Toxin of Clostridium perfringens Type B and D

被引：0
作者：
Javed, Shahzad [1 ]
Rafeeq, Majed [2 ]
Tariq, Mohammad Masood [2 ]
Awan, Muhammad Arif [2 ]
Rashid, Nadeem [2 ]
Ali, Mumtaz [2 ]
机构：
[1] Livestock & Dairy Dev Dept, Quetta, Pakistan
[2] Univ Balochistan, Ctr Adv Studies Vaccinol & Biotechnol, Quetta, Pakistan
来源：
PAKISTAN JOURNAL OF ZOOLOGY | 2012年 / 44卷 / 06期
关键词：
Clostridium perfringens; biochemical characterization; growth characteristics; trypsin hemolysin interaction;
D O I：
暂无
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
The present study was conducted to identify the biochemical characterization, evaluation of growth and hemolytic properties of Clostridium perfringens (C. perfringens) type B and D in different growth media and physical environment. The reinforced clostridial (RC) and thioglycolate (TG) growth media were observed better for the growth and hemolysin production 12 h post-incubation at 37 degrees C (P<0.05). Time, temperature and buffer also illustrated a significant (P<0.05) relationship on the hemolytic activity of proto-toxin of C. perfringens types B and D. The trypsinization of proto-toxin greatly enhanced hemolytic activity and 1% trypsin in physiological saline at 37 degrees C for one hour showed maximum hemolytic units (P<0.05). The reinforced clostridial and thioglycolate growth media were found better for the cultivation of C. perfringens and enhanced hemolytic activity with 1% trypsinization. Present results could be helpful in the preparation of an effective bivalent vaccine against enterotoxaemia and lamb dysentery disease of sheep and goat.
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页码：1575 / 1580
页数：6
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