Development and Validation of a Uplc-Qtof-Ms Method for Blood Analysis of Isomeric Amphetamine-Related Drugs

被引：3

作者：

Alamir, Ahmad ^{[1
,2
]}

Watterson, James ^{[3
]}

Attafi, Ibraheem ^{[1
]}

机构：

[1] Minist Hlth, Poison Control & Med Forens Chem Ctr, Jazan Hlth Affairs, Jazan 45142, Saudi Arabia

[2] Laurentian Univ, Dept Chem & Biochem, Sudbury, ON P3E 2C6, Canada

[3] Laurentian Univ, Dept Forens Sci, Sudbury, ON P3E 2C6, Canada

来源：

SEPARATIONS | 2022年 / 9卷 / 10期

关键词：

amphetamine-related drugs; forensic toxicology; blood; UPLC-qTOF-MS; MMSPE; validation; SWGTOX;

D O I：

10.3390/separations9100285

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The identification of isomeric drugs is gaining increasing importance in forensics and doping control. Isomers vary in terms of safety, effectiveness, and regulation, particularly for amphetamine-related drugs (ARDs). This study developed and validated a pseudo-isocratic UPLC-qTOF-MS analytical method for the identification of isomeric Amphetamine-related drugs (ARDs) in blood following mixed-mode solid-phase extraction (MMSPE). The procedure requires 250 mu L of blood to achieve a limit of quantification (LOQ) and detection (LOD) of 20 ng/mL for all analytes. In aged animal blood samples, extraction recoveries of 63-90% and matrix effects of 9-21% were observed. Precision and accuracy for all analytes were within 20% and 89-118%, respectively. The analytical method was developed and validated in accordance with the Scientific Working Group for Forensic Toxicology (SWGTOX) Standard. It has acceptable accuracy and precision for use in doping control and forensic toxicology.

引用

页数：16

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