Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments

被引:9
作者
Hajihashemi, Shokoofeh [1 ]
Rajabpoor, Shakiba [2 ]
Djalovic, Ivica [3 ]
机构
[1] Behbahan Khatam Alanbia Univ Technol, Dept Plant Biol, Fac Sci, Behbahan, Khuzestan, Iran
[2] Payame Noor Univ, Dept Biol, POB 19395-3697, Tehran, Iran
[3] Inst Field & Vegetable Crops, Dept Maize, M Gorki 30, Novi Sad 21000, Serbia
关键词
Callus; Gibberellin; Paclobutrazol; Polyethylene glycol; Stevia rebaudiana; PROLINE ACCUMULATION; DROUGHT STRESS; GLUTATHIONE; ASCORBATE; REDUCTASE; GROWTH; LEAVES; OXYGEN; ASSAYS;
D O I
10.1007/s12298-017-0498-8
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The study on Stevia callus has the potential to advance the knowledge of antioxidant mechanisms involved in unorganized cells response to drought stress. The effects of polyethylene glycol (PEG; 0 and 4% w/v) in combination with paclobutrazol (PBZ; 0 and 2 mg l(-1)) and gibberellin (GA; 0 and 2 mg l(-1)) were studied on Stevia rebaudiana callus. PEG treatment led to an oxidative stress, as indicated by increased H2O2 content whose accumulation was prevented with PBZ and GA treatments. All treatments of PEG, PBZ and GA increased the total antioxidant capacity, with the highest antioxidant power in PBZ and GA treatments without PEG. The activity of superoxide dismutase, catalase and ascorbate peroxidase significantly increased in PEG treatment alone or in combination with PBZ and GA. All treatments of PEG, PBZ and GA significantly increased proteins, amino acids and proline contents, with the highest increase in presence of PBZ in medium culture. In contrary to proline, the activity of pyrroline-5-carboxylate synthetase and proline dehydrogenase did not change in response to any of the treatments. Collectively, our results demonstrated that PBZ and GA increased reactive oxygen species scavenging and osmolytes in PEG-treated calli more than PEG treatment alone to alleviate negative effects of PEG on Stevia calli. These findings will enable us to design effective genetic engineering strategies in callus culture to generate some somaclonal variation that may be useful in enhancing drought resistance in Stevia.
引用
收藏
页码:335 / 341
页数:7
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