miR-615-5p is restrictedly expressed in cirrhotic and cancerous liver tissues and its overexpression alleviates the tumorigenic effects in hepatocellular carcinoma

被引：59

作者：

El Tayebi, H. M. ^{[1
]}

Hosny, K. A. ^{[2
]}

Esmat, G. ^{[3
]}

Breuhahn, K. ^{[4
]}

Abdelaziz, Ahmed Ihab ^{[1
]}

机构：

[1] German Univ, Mol Pathol Res Grp, Dept Pharmacol & Toxicol, Fac Pharm & Biotechnol, Cairo, Egypt

[2] Cairo Univ, Dept Gen Surg, Fac Med, Cairo, Egypt

[3] Cairo Univ, Dept Endem Med & Hepatol, Cairo, Egypt

[4] Univ Heidelberg Hosp, Inst Pathol, D-69120 Heidelberg, Germany

来源：

FEBS LETTERS | 2012年 / 586卷 / 19期

关键词：

microRNA-615-5p; Heptocellular carcinoma; Proliferation; Migration; GROWTH-FACTOR-II; TUMOR-CELLS; PATHWAY; MICRORNAS; MIGRATION; SURVIVAL; INVASION;

D O I：

10.1016/j.febslet.2012.06.054

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

microRNAs aberrant behavior in heptocellular carcinoma (HCC) plays a major role in HCC pathogenesis. miR-615-5p expression has never been evaluated in HCC. We showed that miR-615-5p was preferentially expressed in HCC, cirrhotic liver tissues and HCC cell lines, but undetected in normal livers. Forced miR-615-5p expression in HCC cell lines led to significant decrease in cell growth and migration. In-silico predication revealed insulin-like growth factor-II (IGF-II) as a potential downstream target for miR-615-5p. Forcing the expression of miR-615-5p showed downregulation of IGF-II mRNA, as well as inhibition of the luciferase activity in a luciferase reporter vector harboring the IGF-II-3'UTR target sequence. miR-615-5p acts as tumor-suppressor in HCC through targeting IGF-II. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3309 / 3316

页数：8

共 25 条

[1] Harnessing the RNA interference pathway to advance treatment and prevention of hepatocellular carcinoma [J].