Preparation of leukodepleted platelet concentrates from pooled buffy coats:: Prestorage filtration with Autostop™BC

被引:24
作者
Pietersz, RNI
van der Meer, PF
Steneker, I
Hinloopen, B
Dekker, WJA
van Zanten, AP
Reesink, HW
机构
[1] Red Cross Blood Bank, NL-1006 AC Amsterdam, Netherlands
[2] Slotervaart Hosp, Amsterdam, Netherlands
关键词
D O I
10.1159/000031057
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background and Objectives: Our requirements for leukocyte-depleted platelet concentrates (LD-PC) for an adult patient are: platelets >240X10(9), leukocytes <5x10(6), volume of 150-400 ml; and at the end of storage a pH between 6.8 and 7.4 and presence of the swirling effect. Our aim was to develop a standardized, semiautomated method for the production of LD-PC, by pooling of buffy coats (BC), and prestorage leukoreduction by filtration. Materials and Methods: Whole blood was collected in Top and Bottom systems, and separated automatically with the Compomat(TM) G3 equipment into a red cell concentrate, a plasma and a BC. Subsequently, a pool of 5 BC was made, and 200 g plasma from one of the donors was added. Then, after soft spin centrifugation, the platelet rich plasma was leukocyte depleted by filtration using the Autostop(TM) BC filter, and stored in a 1,000 ml polyolefin platelet storage bag. Results: BC (n = 60) had a volume of 51+/-2 ml (mean +/- SD) with a hematocrit of 0.44+/-0.03 l/l and contained 80+/-5% of the platelets and 74+/-12% of the leukocytes of the whole blood. Rou tinely prepared LD-PC (n = 15,037) contained a median of 341x10(9) platelets (range 49-599x10(9)), with only 104/15,037 (0.7%) containing fewer than 240x10(9) platelets; the median volume was 263 ml (range 134-373 ml). In 118/917 (13%) LD-PC leukocytes were observed in the Nageotte hemocytometer, but only twice exceeding 1x10(6) leukocytes per unit, and none exceeding 5X10(6) (median <0.6x10(6); range <0.6-1.41x10(6)). Storage experiments of the LD-PC (n = 12) revealed adequate oxygenation and maintenance of pH and swirling effect up to 9 days. Conclusions: This method warrants with 99% confidence that LD-PC contain more than 240X10(9) platelets; with 97.5% confidence that 100% of the LD-PC contain <5X10(6) leukocytes, and with 95% confidence that more than 99% of the LD-PC contain fewer than 1x10(6) leukocytes; these LD-PC can be stored satisfactorily for up to 9 days.
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页码:231 / 236
页数:6
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