A novel plastid localization of chalcone synthase in developing grape berry

被引：42

作者：

Tian, Li ^{[1
]}

Wan, Si-Bao ^{[1
]}

Pan, Qiu-Hong ^{[1
]}

Zheng, Yan-Jun ^{[1
]}

Huang, Wei-Dong ^{[1
]}

机构：

[1] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

来源：

PLANT SCIENCE | 2008年 / 175卷 / 03期

基金：

中国国家自然科学基金;

关键词：

subcellular localization; chalcone synthase; grape berry;

D O I：

10.1016/j.plantsci.2008.03.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chalcone synthase (CHS, E.C. 2.3.1.74) is an entrance enzyme of flavonoid metabolism and a critical point to regulation of biosynthesis of different flavonoid compounds that directly contribute to color and monthfeel of grape and wine. In the present experiment, subcellular localization of CHS in developing grape berry was performed via immunogold electron microscopy technique. The result showed that CHS was localized in rough endoplasmic reticulum (ER) and cytoplasm of the skin cells, while few gold particles representing CHS were found on the cell wall. Besides, two novel localized sites of CHS were observed within a cell of developing grape berry, one being the plastid-distributed throughout developmental stages and the other being vacuole-distributed at late developmental stage. It is speculated that these novel localized patterns may relate to abundant and multi-branch flavonoid metabolism in grape berry. This work will provide new insight for the regulation of different branch pathways leading to diverse flavonoid compounds. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：431 / 436

页数：6

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