Insertion of an Alu element in a lncRNA leads to primate-specific modulation of alternative splicing

被引：76

作者：

Hu, Shanshan ^{[1
]}

Wang, Xiaolin ^{[1
]}

Shan, Ge ^{[1
]}

机构：

[1] Univ Sci & Technol China, Sch Life Sci, CAS Ctr Excellence Mol Cell Sci, CAS Key Lab Innate Immun & Chron Dis, Hefei, Peoples R China

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2016年 / 23卷 / 11期

基金：

中国国家自然科学基金;

关键词：

RNA-POLYMERASE-III; 5S RIBOSOMAL-RNA; NONCODING RNAS; CIRCULAR RNAS; POL II; TRANSCRIPTION; EVOLUTION; CHROMATIN; BINDING; BIOGENESIS;

D O I：

10.1038/nsmb.3302

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Noncoding RNAs, mobile elements, and alternative splicing are all critical for the regulation of gene expression. Here we show that a conserved noncoding RNA acquires a new function due to the insertion of a mobile element. We identified a noncoding RNA, termed 5S-OT, which is transcribed from 5S rDNA loci in eukaryotes including fission yeast and mammals. 5S-OT plays a cis role in regulating the transcription of 5S rRNA in mice and humans. In the anthropoidea suborder of primates, an antisense Alu element has been inserted at the 5S-OT locus. We found that in human cells, 5S-OT regulates alternative splicing of multiple genes in trans via Alu/anti-Alu pairing with target genes and by interacting with the splicing factor U2AF65. This trans effect of 5S-OT in splicing might be exploited in biotechnological applications.

引用

页码：1011 / 1019

页数：9

共 37 条

[11] The four dimensions of noncoding RNA conservation [J].