Chromatographic resolution of partially perdeuterated diglucosyldiacylglycerols from Acholeplasma laidlawii

被引：2

作者：

Bergqvist, MHJ

机构：

[1] Banting and Best Dept. of Med. Res., University of Toronto, Toronto

[2] Department of Analytical Chemistry, Stockholm University, Stockholm

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 736卷 / 1-2期

关键词：

Acholeplasma laidlawii; diglucosyldiacylglycerols; glycerols; glucolipids; lipids;

D O I：

10.1016/0021-9673(95)01344-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Diglucosyldiacylglycerol from Acholeplasma laidlawii was isolated by adsorption chromatography and subsequently subjected to molecular species analysis by reversed-phase high-performance liquid chromatography. Twenty-three molecular species were resolved. The bacterium was grown in a medium supplemented with a mixture of oleic acid and perdeuterated palmitic acid, and incorporation of perdeuterated acyl moieties occurred in several molecular species. Short and odd-numbered acyl chains were found in combinations with palmitic acid, perdeuterated palmitic acid and oleic acid. Also, molecular species differing only by perdeuterated and protonated acyl chain content were resolved chromatographically. Separation between diglucosyldiacylglycerol and digalactosyldiacylglycerol isolated from a plant source was achieved with adsorption chromatography. The polarity of diglucosyldiacylglycerol as compared to digalactosyldiacylglycerol is discussed.

引用

页码：83 / 89

页数：7

共 9 条

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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (20)
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