Development of a method to determine carotenoid composition of fresh forages

被引：5

作者：

Cardinault, N. ^{[1
]}

Lyan, B. ^{[2
]}

Doreau, M. ^{[3
]}

Chauveau, B. ^{[3
]}

Rock, E. ^{[2
]}

Grolier, P. ^{[4
]}

机构：

[1] Fac Med, INSERM, UMR U476, F-13005 Marseille, France

[2] INRA, Unite Nutr Humaine, F-63122 St Genes Champanelle, France

[3] INRA, Unite Rech Herbivores, F-63122 St Genes Champanelle, France

[4] Unite Mixte Rech Psychoneuroimmunol Nutr & Genet, F-33077 Bordeaux, France

来源：

CANADIAN JOURNAL OF PLANT SCIENCE | 2008年 / 88卷 / 06期

关键词：

Xanthophylls; carotenoids; fresh forage; HPLC;

D O I：

10.4141/CJPS07133

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Cardinault, N., Lyan, B., Doreau, M., Chauveau, B., Rock, E. and Grolier, P. 2008. Development of a method to determine carotenoid composition of fresh forages. Can. J. Plant Sci. 88: 1057-1064. Due to the limited interest in carotenoids in ruminant diets until recently, analyses of forages are often incomplete, focusing mainly on P-carotene and lutein. Carotenoid composition of green forage from middle mountain meadow was analyzed by HPLC after extraction and elimination of chlorophylls by mild saponification. This method Of analysis uses two C-18 columns in series with a quaternary gradient system. Our method allowed, for the first time, the identification and quantification of several xanthophylls other than lutein (i.e., violaxanthin, antheraxanthin, epilutein) in chlorophyll-free extracts from carotenoid-rich forage. The intra-day (3.5-7.5%) and inter-day (1.2-3.5%) coefficients of variation are Suitable for routine determination of carotenoids in green forage. This method Could also be used in metabolic studies of these micronutrients in ruminants.

引用

页码：1057 / 1064

页数：8

共 40 条

[1] [Anonymous], 1990, OFF METH AN
[2] Reversed-phase gradient high-performance liquid chromatographic procedure for simultaneous analysis of very polar to nonpolar retinoids, carotenoids and tocopherols in animal and plant samples
Barua, AB
Olson, JA
[J]. JOURNAL OF CHROMATOGRAPHY B, 1998, 707 (1-2): : 69 - 79
[3] Improved normal-phase and reversed-phase gradient high-performance liquid chromatography procedures for the analysis of retinoids and carotenoids in human serum, plant and animal tissues
Barua, AB
[J]. JOURNAL OF CHROMATOGRAPHY A, 2001, 936 (1-2) : 71 - 82
[4] Identification and quantification of carotenoids, tocopherols and chlorophylls in commonly consumed fruits and vegetables
Burns, J
Fraser, PD
Bramley, PM
[J]. PHYTOCHEMISTRY, 2003, 62 (06) : 939 - 947
[5] Effects of mountain grassland maturity stage and grazing management on carotenoids in sward and cow's milk
Calderon, Francisco
Tornambe, Gabriele
Martin, Bruno
Pradel, Philippe
Chauveau-Duriot, Beatrice
Noziere, Pierre
[J]. ANIMAL RESEARCH, 2006, 55 (06): : 533 - 544
[6] THE DEVELOPMENT AND APPLICATION OF A CAROTENOID DATABASE FOR FRUITS, VEGETABLES, AND SELECTED MULTICOMPONENT FOODS
CHUGAHUJA, JK
HOLDEN, JM
FORMAN, MR
MANGELS, AR
BEECHER, GR
LANZA, E
[J]. JOURNAL OF THE AMERICAN DIETETIC ASSOCIATION, 1993, 93 (03) : 318 - 323
[7] Identification and quantification of carotenoids including geometrical isomers in fruit and vegetable juices by liquid chromatography with ultraviolet-diode array detection
Cortés, C
Esteve, MJ
Frígola, A
Torregrosa, F
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (08) : 2203 - 2212
[8] Fraser PD, 2000, PLANT J, V24, P551, DOI [10.1046/j.1365-313x.2000.00896.x, 10.1111/j.1365-313X.2000.00896.x]
[9] A fast, reliable and low-cost saponification protocol for analysis of carotenoids in vegetables
Granado, F
Olmedilla, B
Gil-Martinez, E
Blanco, I
[J]. JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2001, 14 (05) : 479 - 489
[10] GUIMARAES A M, 1992, Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, V44, P57

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