Proteomic and metabolic characterization of membrane vesicles derived fromStreptococcus mutansat different pH values

被引：28
作者：
Cao, Yina [1 ,2 ]
Zhou, Yan [1 ,2 ]
Chen, Dongru [1 ,2 ]
Wu, Ruixue [1 ,2 ]
Guo, Lihong [2 ,3 ]
Lin, Huancai [1 ,2 ]
机构：
[1] Sun Yat Sen Univ, Guanghua Sch Stomatol, Dept Prevent Dent, Guangzhou, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Guangzhou, Guangdong, Peoples R China
[3] Sun Yat Sen Univ, Guanghua Sch Stomatol, Dept Endodont, Guangzhou, Guangdong, Peoples R China
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2020年 / 104卷 / 22期
基金：
中国国家自然科学基金;
关键词：
Streptococcus mutans; Membrane vesicles; Proteomic; Metabolomic; Biofilm formation; GRAM-POSITIVE BACTERIA; STREPTOCOCCUS-MUTANS; EXTRACELLULAR VESICLES; PHOSPHOTRANSFERASE SYSTEM; CARIES STATUS; PROTEINS; MECHANISM; GROWTH; BIOGENESIS; COMPONENTS;
D O I：
10.1007/s00253-020-10563-6
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Bacterial membrane vesicles (MVs) are used as a tool for intercellular communication and seem essential for bacterial survival. However, few data are available on MVs generated byStreptococcus mutans, which is the main aetiological agent of dental caries. The present study presents an integrated proteomics and metabolomics analysis of MVs isolated fromS. mutansat initial pH values of 7.5 and 5.5 and explores their function. The results showed thatS. mutansreleases more MVs with smaller diameters under acidic conditions than under neutral conditions. Proteomic analysis showed 344 common vesicular proteins, including various virulence factors. The expressions of 140 individual proteins and 37 metabolites were altered as a result of culturingS. mutansat different pH values. Co-analyses of proteomic and metabolomics data indicated that ABC transporters underwent significant changes under acid pressure. We concluded thatS. mutansproduced MVs at different pH values to carry proteins associated with cariogenesis. Moreover, the alterations ofS. mutansMVs under acid pressure were associated with ABC transporters. These results increase our knowledge ofS. mutansMVs and imply thatS. mutansMVs may play a functional role in carious infection.
引用
收藏
页码：9733 / 9748
页数：16
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