Effect of different tissues and growth hormones on the in vitro propagation from Curculigo orchioides Gaertn., an endangered medicinal Herb in Vietnam

Curculigo orchioides is an endangered medicinal herb belonging to the family Hypoxidaceae. Using MS medium supplemented with TDZ (0.03, 0.05, 0.07, 1 mg/l) and BAP (0.5, 1, 1.5 mg/l) alone or in combination with 0.05 and 0.1 mg/l NAA, in vitro shoot regenerations were tested from three different tissues (rhizome apical tip, rhizome, leaf) of C. orchioides. Result analysis found that shoot regeneration in MS medium supplemented with TDZ (0.07 mg/l) combination with NAA (0.1 mg/l) from rhizome apical tip of C. orchioides was significantly highest compared among treatment. In this treatment, 100 % cultures responded with an average number of 8.67 shoots per explant. Additionally, rooting per shoot regeneration in the half MS medium supplemented with 1.5 mg/l IBA was significantly highest. The rooted plants were successfully transplanted to soil with 100% success. Based on our results, we suggest that the best effect in vitro regeneration of C. orchioides may be to use MS medium supplemented with TDZ (0.07 mg/l) combination with NAA (0.1 mg/l) from rhizome apical tip tissue.