Horseradish peroxidase sol-gel immobilized for chemiluminescence measurements of alkaline-phosphatase activity

被引：42

作者：

Díaz, AN ^{[1
]}

Sánchez, FG ^{[1
]}

Ramos, MC ^{[1
]}

Torijas, MC ^{[1
]}

机构：

[1] Univ Malaga, Fac Ciencias, Dept Quim Analit, E-29071 Malaga, Spain

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2002年 / 82卷 / 2-3期

关键词：

alkaline-phosphatase HRP; enhanced chemiluminescence; biosensor; 2-naphthyl phosphate;

D O I：

10.1016/S0925-4005(01)01003-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

2-Naphthyl phosphate is a pro-enhancer of the luminol-H2O2-horseradish peroxidase reaction. Alkaline-phosphatase hydrolyses the phosphate group and produces 2-naphthol. This compound is an enhancer of the chemiluminescence (CL) reaction. The influence of 2-naphthyl phosphate and the incubation time on the chemiluminescence reaction. have been studied. The horseradish peroxidase (HRP) was immobilized in a sol-gel matrix, obtaining a biosensor for alkaline-phosphatase. The relative standard deviations using free HRP in solution and the immobilized one were 3.2 (n = 4) and 9.3% (n = 3). respectively. The detection limits for alkaline-phosphatase using free and immobilized HR-P were 128 and 106 muU/ml. respectively. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：176 / 179

页数：4

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