Carbon sources and medium pH affects the growth of Withania somnifera (L.) Dunal adventitious roots and withanolide A production

被引：13

作者：

Murthy, H. N. ^{[1
]}

Praveen, N. ^{[2
]}

机构：

[1] Karnatak Univ, Dept Bot, Plant Biotechnol Lab, Dharwad 580003, Karnataka, India

[2] Konkuk Univ, Coll Life & Environm Sci, Dept Appl Biosci, Seoul 143701, South Korea

来源：

NATURAL PRODUCT RESEARCH | 2013年 / 27卷 / 02期

关键词：

adventitious root culture; carbon source; medium pH; sucrose concentration; Withania somnifera (L; ) Dunal; withanolide; SUGARS; REGENERATION; CULTURES;

D O I：

10.1080/14786419.2012.660691

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This work deals with optimisation of adventitious root suspension culture of Withania somnifera (L.) Dunal (Solanaceae) for the production of biomass and withanolide A and we investigated the effect of carbon source and the initial medium pH on growth and production of withanolide A in adventitious root cultures of Withania somnifera. A 2% sucrose concentration was found to be best for both biomass (113.58gL(1) fresh weight (FW) and 11.33gL(1)dry weight (DW)) and secondary metabolite accumulation (8.93mgg(1) DW) in the tested range of concentration (18%). The biomass of adventitious roots was optimal when the initial medium pH was 5.8 (113.26gL(1) FW and 11.33gL(1) DW) but the withanolide A production was highest at the medium pH level of 5.5 (9.09mgg(1) DW).

引用

页码：185 / 189

页数：5

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