Complete genome analysis of the mandarin fish infectious spleen and kidney necrosis iridovirus

被引:257
作者
He, JG [1 ]
Deng, M
Weng, SP
Li, Z
Zhou, SY
Long, QX
Wang, XZ
Chan, SM
机构
[1] Zhongshan Univ, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China
[2] Univ Hong Kong, Dept Zool, Hong Kong, Hong Kong, Peoples R China
关键词
ISKNV; genome; sequence analysis; taxonomic position; phylogeny; cell hypertrophy iridoviruses;
D O I
10.1006/viro.2001.1208
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The nucleotide sequence of the infectious spleen and kidney necrosis virus (ISKNV) genome was determined and found to comprise 111,362 bp with a G+C content of 54.78%. It contained 124 potential open reading frames (ORFs) with coding capacities ranging from 40 to 1208 amino acids. The analysis of the amino acid sequences deduced from the individual ORFs revealed that 35 of the 124 potential gene products of ISKNV show significant homology to functionally characterized proteins of other species. Some of the putative gene products of ISKNV showed significant homologies to proteins in the GenBank/EMBL/DDBJ databases including enzymes and structural proteins involved in virus replication, transcription, protein modification, and virus-host interaction. In addition, one major repeated sequence showing significant homology to the Red Sea bream iridovirus (RSIV) genome was identified. Based on the information obtained from biological properties (including histopathology, tissue tropisms, natural host range, and geographic distribution), physiochemical and physical properties, and genome analysis, we suggest that ISKNV, RSIV, sea bass iridovirus, grouper iridovirus, and African lampeye iridovirus may belong to a new genus of the Iridoviridae family and are tentatively referred to as cell hypertrophy iridoviruses. (C) 2001 Elsevier Science.
引用
收藏
页码:126 / 139
页数:14
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