"Cycliplex PCR" confirmation of Fusobacterium necrophorum isolates from captive wallabies: A rapid and accurate approach

被引：8

作者：

Antiabong, John F. ^{[1
]}

Boardman, Wayne ^{[2
]}

Smith, Ian ^{[2
]}

Brown, Melissa H. ^{[1
]}

Ball, Andrew S. ^{[1
]}

Goodman, Amanda E. ^{[1
]}

机构：

[1] Flinders Univ S Australia, Sch Biol Sci, Adelaide, SA 5001, Australia

[2] Zoos SA, Monarto Zoo, Princes Hwy, Monarto 5254, Australia

来源：

ANAEROBE | 2013年 / 19卷

关键词：

Fusobacterium necrophorum; Isolation and identification; Cycliplex PCR; Supplemented medium; Indole reaction; Captive wallaby; Wildlife; POLYMERASE-CHAIN-REACTION; ENDODONTIC INFECTIONS; MOLECULAR-DETECTION; ANAEROBIC-BACTERIA; LEMIERRES-SYNDROME; IDENTIFICATION; GENES; ANA; NECROBACILLOSIS; ANIMALS;

D O I：

10.1016/j.anaerobe.2012.12.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Isolation and identification of obligate anaerobic bacteria is labour intensive and time consuming. This has led to the increased application of molecular tools to circumvent part of this problem. We report here the development of a rapid, accurate and cost-effective method to isolate and identify Fusobacterium necrophorum species from South Australian wallaby populations using a supplemented medium (BHIRS) in conjunction with a "Cycliplex PCR" method which involves a stepwise-selective amplification of target PCR products. This report demonstrates the complementation of phenotypic characterization by PCR for accurate and fast identification of E necrophorum isolates from wildlife origin. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：44 / 49

页数：6

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