1-Methylthio-D-xylulose 5-Phosphate Methylsulfurylase: A Novel Route to 1-Deoxy-D-xylulose 5-Phosphate in Rhodospirillum rubrum

被引：13

作者：

Warlick, Benjamin P. ^{[1
,3
]}

Evans, Bradley S. ^{[3
]}

Erb, Tobias J. ^{[1
,3
]}

Ramagopal, Udipi A. ^{[5
]}

Sriram, Jaya ^{[4
]}

Imker, Heidi J. ^{[3
]}

Sauder, J. Michael ^{[6
]}

Bonanno, Jeffrey B. ^{[5
]}

Burley, Stephen K. ^{[6
]}

Tabita, F. Robert ^{[4
]}

Almo, Steven C. ^{[5
]}

Sweedler, Jonathan S. ^{[2
,3
]}

Gerlt, John A. ^{[1
,2
,3
]}

机构：

[1] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[3] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA

[4] Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA

[5] Albert Einstein Coll Med, New York SGX Res Ctr Struct Genom, Dept Biochem, Bronx, NY 10461 USA

[6] Lilly Biotechnol Ctr, San Diego, CA 92121 USA

来源：

BIOCHEMISTRY | 2012年 / 51卷 / 42期

基金：

美国国家卫生研究院;

关键词：

RUBISCO-LIKE PROTEINS;

D O I：

10.1021/bi301215g

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-D-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylthio-D-xylulose 5-phosphate (MTXu 5-P) and reductively dethiomethylated to 1-deoxy-D-xylulose 5-phosphate (DXP), an intermediate in the nonmevalonate isoprenoid pathway [Erb, T. J., et al. (2012) Nat. Chem. Biol., in press]. Dethiomethylation, a novel route to DXP, is catalyzed by MTXu 5-P methylsulfurylase. An active site Cys displaces the enolate of DXP from MTXu 5-P, generating a methyl disulfide intermediate.

引用

页码：8324 / 8326

页数：3

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