Connexin-43: A possible mediator of heat stress effects on ram Sertoli cells

被引:0
|
作者
Hassanpour, Hossain [1 ]
Kadivar, Ali [2 ,3 ]
Mirshokraei, Pejman [1 ,4 ]
Nazari, Hassan [1 ]
Afzali, Azita [1 ]
Badisanaye, Maryam [5 ]
机构
[1] Shahrekord Univ, Res Inst Anim Embryo Technol, Shahrekord, Iran
[2] Shahrekord Univ, Dept Clin Sci, Fac Vet Med, Shahrekord, Iran
[3] Shahrekord Univ, Biotechnol Res Inst, Shahrekord, Iran
[4] Ferdowsi Univ Mashhad, Sch Vet Med, Dept Clin Sci, Mashhad, Iran
[5] Shahrekord Univ, Dept Basic Sci, Fac Vet Med, Shahrekord, Iran
关键词
Connexin-43; Heat stress; Ram; Sertoli cell;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Sertoli cells are an essential group of cells in seminiferous epithelium which provide nutritional and structural supports for spermatogenic cells via cell junctions. In this study, the gene expression of connexin-43, the most abundantly distributed gap junction protein of cells, was investigated in ram Sertoli cells under mild and severe heat stresses with real-time quantitative PCR. Sertoli cells were isolated from testes of 10 lambs. After culture and 3 passages, they were treated with mild (39 degrees C) and severe (42 degrees C) heat stress for 6 hr. The results showed a significant reduction in the percentage of live cells under severe heat stress compared to the control group (32 degrees C), (p < 0.05). Relative quantification analysis revealed significantly higher (3.80 fold increase) values of connexin-43 transcripts in severely heat stressed group than control group (p < 0.05). It is concluded that challenging Sertoli cells with 42 degrees C heat could threaten their survival, and overexpression of connexin-43 may cause dysfunction of Sertoli cells due to heat stress. These findings can be useful to identify the molecular mechanisms involved in adverse effects of heat stress on male reproduction and enhance our understanding of its pathogenesis. (c) 2015 Urmia University. All rights reserved.
引用
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页码:125 / 130
页数:6
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