124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics

被引：82

作者：

Wade, Orsolya K. ^{[1
,2
,3
]}

Woehrstein, Johannes B. ^{[1
,2
,3
]}

Nickels, Philipp C. ^{[1
,2
,3
]}

Strauss, Sebastian ^{[1
,2
,3
]}

Stehr, Florian ^{[3
]}

Stein, Johannes ^{[3
]}

Schueder, Florian ^{[1
,2
,3
]}

Strauss, Maximilian T. ^{[1
,2
,3
]}

Ganji, Mahipal ^{[1
,2
,3
]}

Schnitzbauer, Joerg ^{[1
,2
,3
]}

Grabmayr, Heinrich ^{[1
,2
,3
]}

Yin, Peng ^{[4
,5
]}

Schwille, Petra ^{[3
]}

Jungmann, Ralf ^{[1
,2
,3
]}

机构：

[1] Ludwig Maximilians Univ Munchen, Dept Phys, D-80539 Munich, Germany

[2] Ludwig Maximilians Univ Munchen, Ctr Nanosci, D-80539 Munich, Germany

[3] Max Planck Inst Biochem, D-82152 Martinsried, Germany

[4] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[5] Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA

来源：

NANO LETTERS | 2019年 / 19卷 / 04期

基金：

欧洲研究理事会;

关键词：

Super-resolution microscopy; DNA nanotechnology; DNA-PAINT; barcoding multiplexing; MICROSCOPY; MOLECULES;

D O I：

10.1021/acs.nanolett.9b00508

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach to multiplexed super-resolution microscopy by designing the blinking behavior of targets with engineered binding frequency and duration in DNA-PAINT. We assay this kinetic barcoding approach in silico and in vitro using DNA origami structures, show the applicability for multiplexed RNA and protein detection in cells, and finally experimentally demonstrate 124-plex super-resolution imaging within minutes.

引用

页码：2641 / 2646

页数：6

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