Reaction coupling separation for isosteviol production from stevioside catalyzed by acidic ion-exchange resin

被引：2

作者：

Hu, Xueyi ^{[1
,2
]}

Zhou, Zhuoyu ^{[1
,2
]}

Zhang, Zongying ^{[1
,2
]}

Wang, Xiaoxia ^{[1
,2
]}

Sui, Xiaochen ^{[1
,2
]}

Chen, Junming ^{[1
,2
]}

Xia, Yongmei ^{[1
,2
]}

Zhang, Jue ^{[3
]}

Lin, Jianguo ^{[3
]}

机构：

[1] Jiangnan Univ, Sch Chem & Mat Engn, State Key Lab Food Sci & Technol, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China

[2] Jiangnan Univ, Sch Chem & Mat Engn, Minist Educ, Key Lab Synthet & Biol Colloids, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China

[3] Jiangsu Inst Nucl Med, Minist Hlth, Key Lab Nucl Med, Wuxi 214063, Jiangsu, Peoples R China

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2021年 / 44卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Isosteviol; Stevioside; Hydrolysis; Cation-exchange resin; Anticancer; Ionic liquid; STEVIA-REBAUDIANA BERTONI; DITERPENOID ISOSTEVIOL; DERIVATIVES; MECHANISM;

D O I：

10.1007/s00449-020-02431-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Isosteviol, a prodrug used to be obtained via Wagner-Meerwein rearrangement from steviol with low yield and long reaction time. Herein, an in-situ separation-coupling-reaction is presented to prepare isosteviol from the natural sweetener stevioside. Simply with in-situ water-washing, the product containing 92.98% purity of isosteviol was obtained with a stevioside conversion of 97.23% from a packet bed reactor without further separation. Within the assayed inorganic acid, organic acids and acidic ionic liquids, the acidic ion-exchange resins provided higher product specificity towards isosteviol. Furthermore, comparing to 5-Fluorouracil, the product presented similar and even stronger inhibition on proliferation of the assayed human cancer cells in a time and dose-dependence by causing cell phase arrest. Isosteviol treatment caused G1 arrest on SGC-7901, HCT-8 and HCT-116 cells, S arrest on HepG2, Huh-7 and HepG3B cells, and G2 arrest on MGC-803 cells, respectively. Graphic abstract Reaction coupling separation for isosteviol production catalyzed by acidic ion-exchange resin.

引用

页码：151 / 159

页数：9