Reaction coupling separation for isosteviol production from stevioside catalyzed by acidic ion-exchange resin

被引：2
|
作者：
Hu, Xueyi [1 ,2 ]
Zhou, Zhuoyu [1 ,2 ]
Zhang, Zongying [1 ,2 ]
Wang, Xiaoxia [1 ,2 ]
Sui, Xiaochen [1 ,2 ]
Chen, Junming [1 ,2 ]
Xia, Yongmei [1 ,2 ]
Zhang, Jue [3 ]
Lin, Jianguo [3 ]
机构：
[1] Jiangnan Univ, Sch Chem & Mat Engn, State Key Lab Food Sci & Technol, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China
[2] Jiangnan Univ, Sch Chem & Mat Engn, Minist Educ, Key Lab Synthet & Biol Colloids, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China
[3] Jiangsu Inst Nucl Med, Minist Hlth, Key Lab Nucl Med, Wuxi 214063, Jiangsu, Peoples R China
来源：
BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2021年 / 44卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Isosteviol; Stevioside; Hydrolysis; Cation-exchange resin; Anticancer; Ionic liquid; STEVIA-REBAUDIANA BERTONI; DITERPENOID ISOSTEVIOL; DERIVATIVES; MECHANISM;
D O I：
10.1007/s00449-020-02431-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Isosteviol, a prodrug used to be obtained via Wagner-Meerwein rearrangement from steviol with low yield and long reaction time. Herein, an in-situ separation-coupling-reaction is presented to prepare isosteviol from the natural sweetener stevioside. Simply with in-situ water-washing, the product containing 92.98% purity of isosteviol was obtained with a stevioside conversion of 97.23% from a packet bed reactor without further separation. Within the assayed inorganic acid, organic acids and acidic ionic liquids, the acidic ion-exchange resins provided higher product specificity towards isosteviol. Furthermore, comparing to 5-Fluorouracil, the product presented similar and even stronger inhibition on proliferation of the assayed human cancer cells in a time and dose-dependence by causing cell phase arrest. Isosteviol treatment caused G1 arrest on SGC-7901, HCT-8 and HCT-116 cells, S arrest on HepG2, Huh-7 and HepG3B cells, and G2 arrest on MGC-803 cells, respectively. Graphic abstract Reaction coupling separation for isosteviol production catalyzed by acidic ion-exchange resin.
引用
收藏
页码：151 / 159
页数：9
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