Site-specific binding of chelerythrine and sanguinarine to single pyrimidine bulges in hairpin DNA

被引：31

作者：

Bai, Li-Ping ^{[1
]}

Cai, Zongwei ^{[2
]}

Zhao, Zhong-Zhen ^{[1
]}

Nakatani, Kazuhiko ^{[3
]}

Jiang, Zhi-Hong ^{[1
]}

机构：

[1] Hong Kong Baptist Univ, Sch Chinese Med, Kowloon Tong, Hong Kong, Peoples R China

[2] Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China

[3] Osaka Univ, Inst Sci & Ind Res SANKEN, Dept Regulatory Bioorgan Chem, Ibaraki 5670047, Japan

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2008年 / 392卷 / 04期

关键词：

chelerythrine; sanguinarine; bulge DNA; fluorescence spectrometry; ESI-MS;

D O I：

10.1007/s00216-008-2302-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Spectrofluorometric titration, electrospray ionization time-of-flight mass spectrometric and UV melting methods were employed to study the binding of chelerythrine and sanguinarine to bulged DNA. The results showed that both alkaloids bind specifically to single pyrimidine (C, T) bulge sites. The ability of sanguinarine to bind to both regular and bulged hairpins was found to be stronger than that of chelerythrine, but the binding selectivity of chelerythrine toward single-base bulges was much larger than that of sanguinarine.

引用

页码：709 / 716

页数：8

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