Facing Current Quantification Challenges in Protein Microarrays

被引:12
|
作者
Wellhausen, Robert [1 ,2 ,3 ]
Seitz, Harald [1 ,2 ]
机构
[1] Fraunhofer Inst Biomed Engn IBMT, D-14476 Potsdam, Germany
[2] Max Planck Inst Mol Genet, Dept Vertebrate Genom, D-14195 Berlin, Germany
[3] Free Univ Berlin, Inst Chem & Biochem, D-14195 Berlin, Germany
来源
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2012年
关键词
EXPRESSION; TISSUE;
D O I
10.1155/2012/831347
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The proteome is highly variable and differs from cell to cell. The reasons are posttranslational modifications, splice variants, and polymorphisms. Techniques like next-generation sequencing can only give an inadequate picture of the protein status of a cell. Protein microarrays are able to track these changes on the level they occur: the proteomic level. Therefore, protein microarrays are powerful tools for relative protein quantification, to unveil new interaction partners and to track posttranslational modifications. This papers gives an overview on current protein microarray techniques and discusses recent advances in relative protein quantification.
引用
收藏
页数:8
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