Temporal expression and DNA hypomethylation profile of CD30 in Marek's disease virus-infected chicken spleens

被引：8

作者：

Li, Kaiyang ^{[1
]}

Lian, Ling ^{[1
]}

Yang, Ning ^{[1
]}

Qu, Lujiang ^{[1
]}

机构：

[1] China Agr Univ, Coll Anim Sci & Technol, Dept Anim Genet & Breeding, Natl Engn Lab Anim Breeding, Beijing 100193, Peoples R China

来源：

POULTRY SCIENCE | 2015年 / 94卷 / 06期

基金：

中国国家自然科学基金;

关键词：

chicken; Marek's disease; CD30; gene expression; methylation; FEATHER FOLLICLE EPITHELIUM; LARGE-CELL LYMPHOMA; GENES; METHYLATION; RESISTANT; MODEL; INDUCTION; MEQ;

D O I：

10.3382/ps/pev100

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Marek's disease (MD) is a viral neoplastic disease of chickens caused by Marek's disease virus (MDV), which is serious threat to worldwide poultry industry. Our previous studies showed that the CD30 gene was hypomethylated in MD lymphoma. In this study, we further analyzed differential expression patterns and methylation levels of the CD30 gene between MDV-infected and noninfected spleens at 4, 7, 14, 21, and 28 d postinfection (dpi). The results showed that the expression of CD30 in MDV-infected spleens was significantly lower than that in noninfected spleens at 4 dpi. The expression of CD30 did not present significant difference between MDV-infected and noninfected spleens at 7 and 14 dpi. However, an increased expression of CD30 was presented in MDV-infected spleens at both 21 and 28 dpi. Simultaneously, CD30 showed a lower DNA methylation level in MDV-infected spleens at 14, 21, and 28 dpi. The results indicated that CD30 gene was involved in the whole process of MD tumorigenesis and upregulated expression of CD30 in MDV-infected spleens might be attributed to the hypomethylation of promoter of CD30 gene.

引用

页码：1165 / 1169

页数：5

共 29 条

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