Genomic characterization of emergent pseudorabies virus in China reveals marked sequence divergence: Evidence for the existence of two major genotypes

被引：105

作者：

Ye, Chao ^{[1
]}

Zhang, Qing-Zhan ^{[2
]}

Tian, Zhi-Jun ^{[1
]}

Zheng, Hao ^{[2
]}

Zhao, Kuan ^{[1
]}

Liu, Fei ^{[2
]}

Guo, Jin-Chao ^{[1
]}

Tong, Wu ^{[2
]}

Jiang, Cheng-Gang ^{[1
]}

Wang, Shu-Jie ^{[1
]}

Shi, Mang ^{[3
,4
]}

Chang, Xiao-Bo ^{[1
]}

Jiang, Yi-Feng ^{[2
]}

Peng, Jin-Mei ^{[1
]}

Zhou, Yan-Jun ^{[2
]}

Tang, Yan-Dong ^{[1
]}

Sun, Ming-Xia ^{[1
]}

Cai, Xue-Hui ^{[1
]}

An, Tong-Qing ^{[1
]}

Tong, Guang-Zhi ^{[2
]}

机构：

[1] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China

[2] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China

[3] Univ Sydney, Sch Biol Sci, Sydney Emerging Infect & Biosecur Inst, Sydney, NSW 2006, Australia

[4] Univ Sydney, Sydney Med Sch, Sydney, NSW 2006, Australia

来源：

VIROLOGY | 2015年 / 483卷

基金：

中国国家自然科学基金;

关键词：

PRV; Divergence; RFLP; Microsatellite; Genotype; VARICELLA-ZOSTER-VIRUS; AUJESZKYS-DISEASE; INSERTION/DELETION POLYMORPHISM; MOLECULAR CHARACTERIZATION; WILD BOAR; GENE; DNA; IDENTIFICATION; HERPESVIRUS-1; STRAINS;

D O I：

10.1016/j.virol.2015.04.013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recently pseudorabies outbreaks have occurred in many vaccinated farms in China. To identify genetic characteristics of pseudorabies virus (PRV) strains, we obtained the genomic sequences of PRV strains HeN1 and JS, which were compared to 4 PRV genomes and 729 partial gene sequences. PRV strains isolated in China showed marked sequence divergence compared to European and American strains. Phylogenetic analysis revealed that for the first time PRV can be divided into 2 distinct clusters, with Chinese strains being genotype II and PRVs isolated from other countries being genotype I. Restriction fragment length polymorphism analysis confirmed differences between HeN1 and Bartha strains, as did the presence of unique insertion/deletion polymorphisms and microsatellites. This divergence between the two genotypes may have been generated from long-term, independent evolution, which could also explain the low efficacy of the Bartha vaccine in protecting pigs infected with genotype II PRV. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：32 / 43

页数：12