Determination of polyphenol components of Lonicera japonica Thunb. using liquid chromatography-tandem mass spectrometry: Contribution to the overall antioxidant activity

被引：85

作者：

Seo, On Nuri ^{[2
,3
]}

Kim, Gon-Sup ^{[3
,4
]}

Park, Semin ^{[2
,3
]}

Lee, Jung Han ^{[2
,3
]}

Kim, Yun-Hi ^{[2
,3
]}

Lee, Won Sup ^{[1
,5
]}

Lee, Soo Jung ^{[6
]}

Kim, Chi Yeon ^{[7
]}

Jin, Jong Sung ^{[8
]}

Choi, Sang Kyung ^{[9
]}

Shin, Sung Chul ^{[2
,3
]}

机构：

[1] Gyeongsang Natl Univ, Dept Internal Med, Inst Hlth Sci, Jinju 660702, South Korea

[2] Gyeongsang Natl Univ, Dept Chem, Jinju 660701, South Korea

[3] Gyeongsang Natl Univ, Life Sci Res Inst, Jinju 660701, South Korea

[4] Gyeongsang Natl Univ, Coll Vet Med, Jinju 660701, South Korea

[5] Gyeongsang Natl Univ, Gyeongnam Reg Canc Ctr, Jinju 660701, South Korea

[6] Gyeongsang Natl Univ, Inst Agr & Life Sci, Dept Food & Nutr, Jinju 660701, South Korea

[7] Gyeongsang Natl Univ Hosp, Inst Hlth Sci, Dept Dermatol, Jinju 660702, South Korea

[8] Korea Basic Sci Inst KBSI, Busan Ctr, Div High Technol Mat Res, Pusan 618230, South Korea

[9] Gyeongsang Natl Univ Hosp, Dept Surg, Jinju 660880, South Korea

来源：

FOOD CHEMISTRY | 2012年 / 134卷 / 01期

关键词：

Polyphenols; Flavonoids; Lonicera japonica Thunb; HPLC-MS/MS; Antioxidant activity; Metabolomes; Hydroxycinnamic acid; PHENOLIC-ACIDS; FLAVONOIDS; L; QUANTIFICATION; IDENTIFICATION; CONSTITUENTS; DISEASES; COCOA; MS/MS;

D O I：

10.1016/j.foodchem.2012.02.124

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Three different mixtures of polyphenol components were isolated from the leaf, flower and stem of Lonicera japonica Thunb. via aqueous 70% methanol extraction followed by column chromatography over silica gel. The polyphenolic components of these mixtures were analysed via high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) and compared with the reported data. Among the 25 characterized components, seven hydroxycinnamic acid derivatives and four flavonoid constituents were identified for the first time. The polyphenol compounds were validated and quantified on the basis of a representative polyphenol standard of the same group. The antioxidant activity of the polyphenol mixtures of the three tissues was determined. The antioxidant activity of the leaf was higher than that of the flower and stem. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：572 / 577

页数：6

共 27 条

[1]

Borek C., 1993, ENVIRON HEALTH PERSP, V101, P151

[2] Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae) [J].