Long noncoding RNAs expression profile and long noncoding RNA-mediated competing endogenous RNA network in nonobstructive azoospermia patients

被引：22
作者：
Bo, Hao [1 ,2 ,3 ]
Liu, Zhizhong [1 ,2 ]
Zhu, Fang [2 ]
Zhou, Dai [2 ,3 ]
Tan, Yueqiu [2 ,3 ]
Zhu, Wenbing [2 ,3 ]
Fan, Liqing [2 ,3 ]
机构：
[1] Cent South Univ, Hunan Canc Hosp, Affiliated Canc Hosp, Dept Urol,Xiangya Sch Med, Changsha, Peoples R China
[2] Cent South Univ, Key Lab Human Stem Cells & Reprod, Minist Hlth, Sch Basic Med Sci,Inst Reprod & Stem Cell Engn, Changsha, Peoples R China
[3] Reprod & Genet Hosp CITIC Xiangya, Dept Sci Res, Changsha, Peoples R China
来源：
EPIGENOMICS | 2020年 / 12卷 / 08期
基金：
中国国家自然科学基金;
关键词：
ceRNA network; expression profile; LINC00467; lncRNAs; NOA; GENOME-WIDE ASSOCIATION; REVEALS; TARGETS; ROLES; RISK;
D O I：
10.2217/epi-2020-0008
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Aim: To analyze the expression profile and competing endogenous RNA (ceRNA) network of long noncoding RNAs (lncRNAs) in nonobstructive azoospermia (NOA). Materials & methods: The lncRNA expression profile in NOA was determined by microarray reanalysis. Differential expression analysis was performed by R software. The ceRNA network was constructed using correlation analysis and gene target miRNA prediction. Metascape was used for enrichment analysis. Again ceRNA network was validated by quantitative real-time PCR. Results: Many lncRNAs are differently expressed in NOA. LncRNAs might participate in spermatogenesis through ceRNA mechanism. The ceRNA network included male gamete generation and other pathways. LINC00467 in the network regulated the expression of LRGUK and TDRD6. Conclusion: LncRNAs are involved in NOA and potential biomarkers and therapeutic targets for NOA.
引用
收藏
页码：673 / 684
页数：12
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