Analytical techniques for single-liposome characterization

被引：46

作者：

Chen, Chaoxiang ^{[1
]}

Zhu, Shaobin ^{[1
]}

Huang, Tianxun ^{[1
]}

Wang, Shuo ^{[1
]}

Yan, Xiaomei ^{[1
]}

机构：

[1] Xiamen Univ, Coll Chem & Chem Engn, Dept Biol Chem, Key Lab Chem Biol Fujian Prov,Key Lab Analyt Sci, Xiamen 361005, Fujian, Peoples R China

来源：

ANALYTICAL METHODS | 2013年 / 5卷 / 09期

基金：

中国国家自然科学基金;

关键词：

TRANSMISSION ELECTRON-MICROSCOPY; NANOPARTICLE TRACKING ANALYSIS; CONFOCAL RAMAN MICROSCOPY; GIANT UNILAMELLAR VESICLES; SENSITIVITY FLOW CYTOMETER; ATOMIC-FORCE MICROSCOPY; PHOSPHOLIPID-VESICLES; SIZE DISTRIBUTION; VERSATILE TOOL; DRUG-DELIVERY;

D O I：

10.1039/c3ay40219c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Liposomes or phospholipid vesicles are one of the most versatile nanoparticles used to convey drugs, vaccines, genes, enzymes, or other substances to target cells and as a model to mimic biological membranes. To fulfil their roles in drug delivery and biotechnology, the physical and chemical properties of liposomes, such as size, shape, chemical composition, lamellarity, encapsulation efficiency of cargo molecules, and the density of proteins reconstituted in the membrane, need to be characterized to ensure reproducible preparation of the vesicles. Compared to bulk analysis, techniques focusing on the individual analysis of liposomes can reveal heterogeneity that is otherwise masked by ensemble averaging. Herein, we review the recent advances in techniques for single-liposome characterization.

引用

页码：2150 / 2157

页数：8

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