Microsatellite loci in the house fly, Musca domestica L. (Diptera: Muscidae)

被引：0
作者：
Endsley, MA [1 ]
Baker, MD [1 ]
Krafsur, ES [1 ]
机构：
[1] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA
来源：
MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 01期
关键词：
microsatellites; Musca autumnalis; Musca domestica; simple sequence repeats;
D O I：
10.1046/j.1471-8278 .2001.00153.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Genomic libraries from house flies enriched for (CA)(15) and (CAG)(10) repeats were constructed by using biotinylated probes. Twenty-five loci were isolated and evaluated for polymorphisms in wild flies representing two geographically diverse populations. Fourteen of 19 dinucleotide loci, and one of six trinucleotide loci were polymorphic. One hundred and twenty-seven alleles were detected, 39 of which were private. Average number of alleles per polymorphic locus was 8.4 +/- 2.5 and average heterozygosity was 72 +/- 4%. F-ST by the private allele method was 0.73. Three of 15 loci showed significant heterozygote deficiencies, attributed to null alleles. Five of 15 loci were amplified in the face fly, Musca autumnalis.
引用
收藏
页码：72 / 74
页数：3
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