Microsatellite loci in the house fly, Musca domestica L. (Diptera: Muscidae)

Genomic libraries from house flies enriched for (CA)(15) and (CAG)(10) repeats were constructed by using biotinylated probes. Twenty-five loci were isolated and evaluated for polymorphisms in wild flies representing two geographically diverse populations. Fourteen of 19 dinucleotide loci, and one of six trinucleotide loci were polymorphic. One hundred and twenty-seven alleles were detected, 39 of which were private. Average number of alleles per polymorphic locus was 8.4 +/- 2.5 and average heterozygosity was 72 +/- 4%. F-ST by the private allele method was 0.73. Three of 15 loci showed significant heterozygote deficiencies, attributed to null alleles. Five of 15 loci were amplified in the face fly, Musca autumnalis.